Punicalagin inhibits neuroinflammation in LPS-activated rat primary microglia
Version of Record online: 28 JUL 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 9, pages 1843–1851, September 2014
How to Cite
Olajide, O. A., Kumar, A., Velagapudi, R., Okorji, U. P. and Fiebich, B. L. (2014), Punicalagin inhibits neuroinflammation in LPS-activated rat primary microglia. Mol. Nutr. Food Res., 58: 1843–1851. doi: 10.1002/mnfr.201400163
- Issue online: 8 SEP 2014
- Version of Record online: 28 JUL 2014
- Manuscript Accepted: 19 MAY 2014
- Manuscript Revised: 17 MAY 2014
- Manuscript Received: 6 MAR 2014
- Alexander von Humboldt Foundation
- University of Huddersfield International Networking Fund
- State Law on Graduate Funding
In this study, the effects of punicalagin on neuroinflammation in LPS-activated microglia were investigated.
Methods and results
The ability of punicalagin to reduce the production of TNF-α, IL-6 and prostaglandin E2 was measured in culture medium using enzyme immunoassay. TNF-α and IL-6 gene expression in mouse hippocampal slices was measured with PCR. cyclooxygenase-2 and microsomal prostaglandin E synthase 1 protein and mRNA were evaluated with Western blotting and PCR, respectively. Further experiments to investigate effects of punicalagin on protein expressions of inflammatory targets were also determined with Western blotting. Pretreatment of rat primary microglia with punicalagin (5–40 μM) prior to LPS (10 ng/mL) stimulation produced a significant (p < 0.05) inhibition of TNF-α, IL-6 and prostaglandin E2 production. Punicalagin completely abolished TNF-α and IL-6 gene expression in LPS-stimulated hippocampal slices. Protein and mRNA expressions of cyclooxygenase-2 and microsomal prostaglandin E synthase 1 were also reduced by punicalagin pretreatment. Results show that punicalagin interferes with NF-κB signalling through attenuation of NF-κB-driven luciferase expression, as well as inhibition of IκB phosphorylation and nuclear translocation of p65 subunit in the microglia.
These results suggest that punicalagin inhibits neuroinflammation in LPS-activated microglia through interference with NF-κB signalling, suggesting its potential as a nutritional preventive strategy in neurodegenerative disorders.