These authors contributed equally to this work.
Alternating or continuous exposure to cafeteria diet leads to similar shifts in gut microbiota compared to chow diet
Version of Record online: 27 MAY 2016
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
How to Cite
Kaakoush, N. O., Martire, S. I., Raipuria, M., Mitchell, H. M., Nielsen, S., Westbrook, R. F. and Morris, M. J. (2016), Alternating or continuous exposure to cafeteria diet leads to similar shifts in gut microbiota compared to chow diet. Mol. Nutr. Food Res.. doi: 10.1002/mnfr.201500815
- Version of Record online: 27 MAY 2016
- Accepted manuscript online: 14 JAN 2016 10:09PM EST
- Manuscript Accepted: 29 DEC 2015
- Manuscript Revised: 15 DEC 2015
- Manuscript Received: 15 OCT 2015
- Binge eating;
- Metabolic disease;
- Western cafeteria diet
Overconsumption of energy-rich food is a major contributor to the obesity epidemic. The eating habits of many people are characterized by the cycling between overconsumption of energy-rich foods and dieting, the effects of which on the microbiota are currently unknown.
Methods and results
We compared the fecal microbiota of rats either continuously fed chow or palatable cafeteria diet to a “cycled” group switched between the two diets (chow for 4, cafeteria for 3 days/wk, n = 12/group) over 16 wk. Enriched bacterial metabolic pathways were predicted, and a range of metabolic parameters was correlated to microbial taxa and pathways. Cycled rats showed large excursions in food intake on each diet switch. When switched from chow to cafeteria, they overconsumed, and when switched back to chow they underconsumed relative to those maintained on the two diets. Metabolic parameters of cycled rats were intermediate between those of the other diet groups (p < 0.05). The microbiota of cycled rats was nearly indistinguishable from rats under constant cafeteria diet, and both groups were significantly different to the chow group. Correlation analyses identified microbial metabolic pathways associated with an obese phenotype.
These data suggest that continuous or intermittent exposure to palatable foods have similar effects on the gut microbiota.