Research Article

Insights into cyclodextrin interactions during sample stacking using capillary isotachophoresis with on-line microcoil NMR detection†

  1. Valentino K. Almeida1,
  2. Cynthia K. Larive2,*

Article first published online: 27 JUL 2005

DOI: 10.1002/mrc.1626

Issue

Magnetic Resonance in Chemistry

Magnetic Resonance in Chemistry

Special Issue: Hyphenated NMR Techniques, Guest Editor: Professor Klaus Albert, University of Tübingen, Germany

Volume 43, Issue 9, pages 755–761, September 2005

Additional Information

How to Cite

Almeida, V. K. and Larive, C. K. (2005), Insights into cyclodextrin interactions during sample stacking using capillary isotachophoresis with on-line microcoil NMR detection. Magnetic Resonance in Chemistry, 43: 755–761. doi: 10.1002/mrc.1626

Author Information

  1. 1

    Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA

  2. 2

    Department of Chemistry, University of California–Riverside, Riverside, CA 92521, USA

*Department of Chemistry, University of California–Riverside, Riverside, CA 92521, USA.

  1. †

    Presented as part of a special issue on Hyphenated NMR Techniques

Publication History

  1. Issue published online: 27 JUL 2005
  2. Article first published online: 27 JUL 2005
  3. Manuscript Accepted: 18 MAY 2005
  4. Manuscript Revised: 13 MAY 2005
  5. Manuscript Received: 11 OCT 2004

Funded by

  • NSF. Grant Number: CHE 0213407.

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Keywords:

  • microcoil NMR;
  • capillary isotachophoresis;
  • inclusion complex

Abstract

On-line capillary isotachophoresis (cITP)–NMR experiments were used to probe the interactions of the pharmaceutical compounds S-alprenolol, S-atenolol, R-propranolol, R-salbutamol and S-terbutaline with β-cyclodextrin (β-CD) during cITP concentration. In cITP, ionic analytes are concentrated and separated on the basis of their electrophoretic mobility. Because neutral molecules have an electrophoretic mobility of zero, they are normally not concentrated or separated in electrophoretic experiments like cITP. Most of the analytes studied were concentrated by cITP sample stacking by a factor of around 300. For analytes that formed a strong inclusion complex, β-CD co-concentrated during cITP sample stacking. However, once the focusing process was complete, a discrete diffusional boundary formed between the cITP-focused analyte band and the leading and trailing electrolyte, which restricted diffusion into and out of the analyte band. Copyright © 2005 John Wiley & Sons, Ltd.

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