Review

LC–NMR coupling technology: recent advancements and applications in natural products analysis†

  1. Vassiliki Exarchou1,*,
  2. Manfred Krucker2,
  3. Teris A. van Beek3,
  4. Jacques Vervoort4,
  5. Ioannis P. Gerothanassis5,
  6. Klaus Albert2

Article first published online: 27 JUL 2005

DOI: 10.1002/mrc.1632

Issue

Magnetic Resonance in Chemistry

Magnetic Resonance in Chemistry

Special Issue: Hyphenated NMR Techniques, Guest Editor: Professor Klaus Albert, University of Tübingen, Germany

Volume 43, Issue 9, pages 681–687, September 2005

Additional Information

How to Cite

Exarchou, V., Krucker, M., van Beek, T. A., Vervoort, J., Gerothanassis, I. P. and Albert, K. (2005), LC–NMR coupling technology: recent advancements and applications in natural products analysis. Magnetic Resonance in Chemistry, 43: 681–687. doi: 10.1002/mrc.1632

Author Information

  1. 1

    NMR Center, Department of Chemistry, University of Ioannina, Ioannina, GR-45110, Greece

  2. 2

    Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 18, D-72076 Tübingen, Germany

  3. 3

    Laboratory of Organic Chemistry, Natural Products Chemistry Group, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands

  4. 4

    Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands

  5. 5

    Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina GR-45110, Greece

*NMR Center, Department of Chemistry, University of Ioannina, Ioannina, GR-45110, Greece.

  1. †

    Presented as part of a special issue on Hyphenated NMR Techniques

Publication History

  1. Issue published online: 27 JUL 2005
  2. Article first published online: 27 JUL 2005
  3. Manuscript Accepted: 18 MAY 2005
  4. Manuscript Revised: 11 MAY 2005
  5. Manuscript Received: 12 JAN 2005

Funded by

  • European Union project. Grant Number: HPRI-CT-1999-50018.
  • Deutsche Forschungsgemeinschaft. Grant Number: AL 298/10-1.
  • Programmme for the Promotion of the Exchange and Scientific Cooperation between Greece and Germany. Grant Number: Program IKYDA 2002.
  • Program ‘Pythagoras’ of the Operational Program for Education and Initial Vocational Training of the Hellenic Ministry of Education.
  • European Social Fund.

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (143K)

Keywords:

  • LC–NMR;
  • LC–SPE–NMR;
  • hyphenation;
  • natural products

Abstract

An overview of recent advances in nuclear magnetic resonance (NMR) coupled with separation technologies and their application in natural product analysis is given and discussed. The different modes of LC–NMR operation are described, as well as how technical improvements assist in establishing LC–NMR as an important tool in the analysis of plant-derived compounds. On-flow, stopped-flow and loop-storage procedures are mentioned, together with the new LC–SPE–NMR configuration. The implementation of mass spectrometry in LC–NMR is also useful on account of the molecular weight and fragmentation information that it provides, especially when new plant species are studied. Cryogenic technology and capillary LC–NMR are the other important recent developments. Since the plant kingdom is endless in producing potential drug candidates, development and optimization of LC–NMR techniques convert the study of natural products to a less-time-consuming task, speeding up identification. Copyright © 2005 John Wiley & Sons, Ltd.

Get PDF (143K)