Research Article

J-based 2D homonuclear and heteronuclear correlation in solid-state proteins

  1. Lingling Chen1,
  2. J. Michael Kaiser1,
  3. Jinfeng Lai1,
  4. Tatyana Polenova2,
  5. Jun Yang2,
  6. Chad M. Rienstra3,
  7. Leonard J. Mueller1,*

Article first published online: 21 DEC 2007

DOI: 10.1002/mrc.2107

Issue

Magnetic Resonance in Chemistry

Magnetic Resonance in Chemistry

Special Issue: New techniques in solid-state NMR

Volume 45, Issue S1, pages S84–S92, December 2007

Additional Information

How to Cite

Chen, L., Kaiser, J. M., Lai, J., Polenova, T., Yang, J., Rienstra, C. M. and Mueller, L. J. (2007), J-based 2D homonuclear and heteronuclear correlation in solid-state proteins. Magn. Reson. Chem., 45: S84–S92. doi: 10.1002/mrc.2107

Author Information

  1. 1

    Department of Chemistry, University of California, Riverside, California 92521, USA

  2. 2

    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA

  3. 3

    Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

*Department of Chemistry, University of California, Riverside, CA 92521, USA.

Publication History

  1. Issue published online: 21 DEC 2007
  2. Article first published online: 21 DEC 2007
  3. Manuscript Accepted: 17 SEP 2007
  4. Manuscript Revised: 9 SEP 2007
  5. Manuscript Received: 30 JUL 2007

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Keywords:

  • NMR;
  • 13C;
  • 15N;
  • solid-state NMR;
  • scalar-coupling-driven correlation;
  • through-bond correlation;
  • protein

Abstract

Scalar-based two-dimensional heteronuclear experiments are reported for NCO and NCA chemical shift correlation in the solid state. In conjunction with homonuclear CACO correlation, these experiments form a useful set for tracing connectivities and assigning backbone resonances in solid-state proteins. The applicability of this approach is demonstrated on two proteins, the β 1 immunoglobulin binding domain of protein G at 9.4 T and reassembled thioredoxin at 14.1 T, using different decoupling conditions and MAS frequencies. These constant-time J-based correlation experiments exhibit increased resolution in the indirect dimension owing to homonuclear and heteronuclear decoupling, and because the indirect evolution and transfer periods are combined into a single constant time interval, this increased resolution is not obtained at the cost of sensitivity. These experiments are also shown to be compatible with in-phase anti-phase (IPAP) selection, giving increased resolution in the directly detected dimension. Copyright © 2007 John Wiley & Sons, Ltd.

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