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Research Article
Membrane orientation of the Na,K-ATPase regulatory membrane protein CHIF determined by solid-state NMR
Article first published online: 21 DEC 2007
DOI: 10.1002/mrc.2144
Copyright © 2007 John Wiley & Sons, Ltd.
Issue
1097-458Xa/asset/cover.gif?v=1&s=d5f2b3f6d127fcc37c26f1dbdb0c8887da653c0f "Magnetic Resonance in Chemistry")
Magnetic Resonance in Chemistry
Special Issue: New techniques in solid-state NMR
Volume 45, Issue S1, pages S192–S197, December 2007
Additional Information
How to Cite
Franzin, C. M., Teriete, P. and Marassi, F. M. (2007), Membrane orientation of the Na,K-ATPase regulatory membrane protein CHIF determined by solid-state NMR. Magn. Reson. Chem., 45: S192–S197. doi: 10.1002/mrc.2144
Publication History
- Issue published online: 21 DEC 2007
- Article first published online: 21 DEC 2007
- Manuscript Accepted: 16 OCT 2007
- Manuscript Revised: 4 OCT 2007
- Manuscript Received: 13 JUL 2007
Funded by
- NIH. Grant Number: R01CA082864,P41EB002031, P30CA030199
- Abstract
- References
- Cited By
Keywords:
- CHIF;
- FXYD4;
- Na,K-ATPase;
- structure;
- membrane protein;
- bilayers;
- solid-state NMR
Abstract
Corticosteroid hormone-induced factor (CHIF) is a major regulatory subunit of the Na,K-ATPase, and a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here we present the structure of CHIF oriented in the membrane, determined by solid-state NMR orientation-dependent restraints. Because CHIF adopts a similar structure in lipid micelles and bilayers, it is possible to assign the solid-state NMR spectrum measured for 15N-labeled CHIF in oriented bilayers from the structure determined in micelles, to obtain the global orientation of the protein in the membrane. Copyright © 2007 John Wiley & Sons, Ltd.