Research Article

Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields

  1. Seth A. McNeill1,
  2. Peter L. Gor'kov2,
  3. Jochem Struppe3,
  4. William W. Brey2,
  5. Joanna R. Long1,*

Article first published online: 21 DEC 2007

DOI: 10.1002/mrc.2146

Issue

Magnetic Resonance in Chemistry

Magnetic Resonance in Chemistry

Special Issue: New techniques in solid-state NMR

Volume 45, Issue S1, pages S209–S220, December 2007

Additional Information

How to Cite

McNeill, S. A., Gor'kov, P. L., Struppe, J., Brey, W. W. and Long, J. R. (2007), Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields. Magn. Reson. Chem., 45: S209–S220. doi: 10.1002/mrc.2146

Author Information

  1. 1

    Department of Biochemistry and Molecular Biology, McKnight Brain Institute, Department of Electrical and Computer Engineering, University of Florida, Gainesville, FL 32610, USA

  2. 2

    National High Magnetic Field Laboratory, Tallahassee, FL 32310, USA

  3. 3

    Bruker Biospin Corp., Billerica, MA, USA

*Department of Biochemistry and Molecular Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.

Publication History

  1. Issue published online: 21 DEC 2007
  2. Article first published online: 21 DEC 2007
  3. Manuscript Accepted: 16 OCT 2007
  4. Manuscript Revised: 1 OCT 2007
  5. Manuscript Received: 22 JUN 2007

Funded by

  • NIH. Grant Number: R01 HL076586
  • National High Magnetic Field Laboratory
  • University of Florida

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Keywords:

  • solid-state NMR;
  • membrane proteins;
  • probe design;
  • pulse sequence;
  • sample heating;
  • low-E

Abstract

Solid-state NMR spectroscopy at high magnetic fields is proving to be an effective technique in structural biology, particularly for proteins which are not amenable to traditional X-ray and solution NMR approaches. Several parameters can be selected to provide optimal sensitivity, improve sample stability, and ensure biological relevance for ssNMR measurements on protein samples. These include selection of sample conditions, NMR probe design, and design of pulse experiments. Here, we demonstrate and evaluate several engineering and experimental approaches for pursuing measurements on dilute proteins in heterogeneous mixtures. Copyright © 2007 John Wiley & Sons, Ltd.

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