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Research Article
Using perdeuterated surfactant micelles to resolve mixture components in diffusion-ordered NMR spectroscopy
Article first published online: 12 NOV 2008
DOI: 10.1002/mrc.2348
Copyright © 2008 John Wiley & Sons, Ltd.
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Additional Information
How to Cite
Zielinski, M. E. and Morris, K. F. (2009), Using perdeuterated surfactant micelles to resolve mixture components in diffusion-ordered NMR spectroscopy. Magnetic Resonance in Chemistry, 47: 53–56. doi: 10.1002/mrc.2348
Publication History
- Issue published online: 9 DEC 2008
- Article first published online: 12 NOV 2008
- Manuscript Accepted: 4 SEP 2008
- Manuscript Revised: 28 AUG 2008
- Manuscript Received: 31 JUL 2008
Funded by
- Donors of the American Chemical Society Petroleum Research Fund. Grant Number: 46707-B4
- Abstract
- References
- Cited By
Keywords:
- 1H NMR;
- DOSY;
- PGSE;
- mixture analysis;
- micelle;
- peptide
Abstract
Diffusion-ordered NMR spectroscopy resolves mixture components on the basis of differences in their respective diffusion coefficients or molecular sizes. However, when components have near-identical diffusion coefficients, they are not resolved in the diffusion dimension of a diffusion-ordered spectroscopy (DOSY) spectrum. Adding surfactant micelles to these mixtures has been shown to enhance resolution when the component molecules interact differentially with the micelles. This approach is similar to that used in electrokinetic chromatography (EKC) where modifiers like micelles or polymers are used to enhance the separation of mixture components. In this study, perdeuterated surfactants are added to analyte mixtures studied with the DOSY technique. Since no micelle resonances appear in the mixture spectra, the difficulty associated with performing biexponential analyses in spectral regions where analyte and surfactant resonances overlap is avoided. The approach is demonstrated using mixtures of peptides with near-identical diffusion coefficients. Copyright © 2008 John Wiley & Sons, Ltd.