Dedicated to Jim Alderfer for his pioneering contributions to establish NMR-based metabonomics at the Roswell Park Cancer Institute.
Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1H-NMR micro-flow probe†
Article first published online: 18 AUG 2009
Copyright © 2009 John Wiley & Sons, Ltd.
Magnetic Resonance in Chemistry
Supplement: NMR-based mixture analysis – metabolomics and beyond
Volume 47, Issue S1, pages S81–S85, December 2009
How to Cite
Sukumaran, D. K., Garcia, E., Hua, J., Tabaczynski, W., Odunsi, K., Andrews, C. and Szyperski, T. (2009), Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1H-NMR micro-flow probe. Magn. Reson. Chem., 47: S81–S85. doi: 10.1002/mrc.2469
- Issue published online: 6 NOV 2009
- Article first published online: 18 AUG 2009
- Manuscript Accepted: 27 MAY 2009
- Manuscript Revised: 21 MAY 2009
- Manuscript Received: 22 JAN 2009
- multivariate data analysis;
- micro-flow probe;
- NMR data quality assessment
A standard operating procedure (SOP) is presented for high-throughput metabonomics studies using a 600 MHz NMR spectrometer equipped with a micro-flow probe that is connected to an auto-sampler. The procedure is designed to minimize random and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the data acquired by a given NMR spectroscopist to ensure that (i) all researchers involved in the NMR data acquisition of a metabonomics research program perform equally and (ii) operator-associated variation of NMR data collection is statistically not relevant for the interpretation of results obtained from multivariate data analyses. Copyright © 2009 John Wiley & Sons, Ltd.