Quantification of taxanes in a leaf and twig extract from Taxus baccata L. using 13C NMR spectroscopy


Correspondence to: Mathieu Paoli, Université de Corse, UMR CNRS 6134 SPE, Equipe Chimie et Biomasse, Route des Iles Sanguinaires, 20000 Ajaccio, France. E-mail: mathieu.paoli@univ-corse.fr


In the course of our ongoing work on the chemical characterization of Taxus baccata L. growing wild in Corsica, we have developed and validated a method for direct quantification of taxane derivatives by 13C NMR using 10-deacetylbaccatin III as reference compound and 1,6-hexanediol as internal standard. We have observed good accuracy (relative errors between 0.3% and 3.5%), linearity (R2 = 0.999) and precision (reproducibility 8.5 mg ± 1.1%) of the measurements. The experimental procedure was applied to the quantification of six identified taxanes in a fraction of chromatography of a methanol extract of T. baccata leaves. This method can be applied to other compounds bearing the taxane skeleton. Copyright © 2013 John Wiley & Sons, Ltd.