Metabonomic investigations into the global biochemical sequelae of exposure to the pancreatic toxin 1-cyano-2-hydroxy-3-butene in the rat (pages S26–S35)
Eszter Bohus, Ákos Rácz, Béla Noszál, Muireann Coen, Olaf Beckonert, Hector C. Keun, Timothy M. D. Ebbels, Glenn H. Cantor, John A. Wijsman, Elaine Holmes, John C. Lindon and Jeremy K. Nicholson
Article first published online: 28 JUL 2009 | DOI: 10.1002/mrc.2485
A metabonomic approach was used to reveal the time-related metabolic effects of 1-cyano-2-hydroxy-3-butene (CHB, crambene) exposure in rats using high-resolution 1H NMR spectroscopy of urine and serum in combination with pattern recognition methods. The spectral correlation technique STOCSY was applied to identify CHB metabolites in urine and to detect both pharmacological and toxicological connectivities between xenobiotic and endogenous metabolites for the first time. The approach may be of wider application in drug metabolism studies for identification of unknown toxic species.