Expression of estrogen receptors α and β in the corpus luteum and uterus from non-pregnant and pregnant llamas
Article first published online: 11 JAN 2007
Copyright © 2007 Wiley-Liss, Inc.
Molecular Reproduction and Development
Volume 74, Issue 8, pages 1043–1052, August 2007
How to Cite
Powell, S. A., Smith, B. B., Timm, K. I. and Menino, A. R. (2007), Expression of estrogen receptors α and β in the corpus luteum and uterus from non-pregnant and pregnant llamas. Mol. Reprod. Dev., 74: 1043–1052. doi: 10.1002/mrd.20684
- Issue published online: 16 MAY 2007
- Article first published online: 11 JAN 2007
- Manuscript Accepted: 16 OCT 2006
- Manuscript Received: 14 SEP 2006
Because estrogen may be involved in maternal recognition of pregnancy and embryonic migration in llamas, expression of estrogen receptor subtypes α (ERα) and β (ERβ) was evaluated in corpus luteum (CL), endometrium, and uterus using relative RT-PCR. Tissues were recovered from sterile-mated (SM) and pregnant (PG) females during Days 7–11 and 7–13 (Day 0 = day of mating), respectively, and follicular phase and juvenile females. Luteal expression of ERα and β was similar (P > 0.10) in SM and PG females and within Days 7–11, however, expression of ERα in ovarian tissue from follicular phase females was greater (P < 0.05) than Days 7 and 9 CL. Uterus expressed less ERα and β compared to endometrium (P = 0.07 and P < 0.01, respectively). Expression of ERα was greater (P < 0.05) in Day 7 and follicular phase uteri than Days 9 and 11, Day 13 PG and juvenile uteri. Uterine ERβ expression was greater (P = 0.09) in PG versus SM females and in mated compared to follicular phase females (P < 0.05). Endometrial expression of ERα and β did not differ (P > 0.10) between SM and PG females or by day. The presence of luteal ER during this period may mean a role for estradiol in maternal recognition of pregnancy. Observed increases in uterine ER expression with no changes in endometrium suggest expression increased in myometrium and/or perimetrium. Upregulation of myometrial ERβ in PG females may be involved in supporting uterine migration of the embryo. Mol. Reprod. Dev. 74: 1043–1052, 2007. © 2007 Wiley-Liss, Inc.