Nicotine induces multinuclear formation and causes aberrant embryonic development in bovine
Article first published online: 21 DEC 2007
Copyright © 2007 Wiley-Liss, Inc.
Molecular Reproduction and Development
Volume 75, Issue 5, pages 801–809, May 2008
How to Cite
Liu, Y., Li, G.-P., Sessions, B. R., Rickords, L. F., White, K. L. and Bunch, T. D. (2008), Nicotine induces multinuclear formation and causes aberrant embryonic development in bovine. Mol. Reprod. Dev., 75: 801–809. doi: 10.1002/mrd.20774
- Issue published online: 21 FEB 2008
- Article first published online: 21 DEC 2007
- Manuscript Accepted: 17 APR 2007
- Manuscript Received: 26 JAN 2007
The present study was designed to investigate the effects of nicotine on development of bovine embryos derived from parthenogenetic activation (PA) and in vitro fertilization (IVF). Nicotine caused disfigured secondary meiotic spindle structures and affected embryonic development in a dose-dependent manner. Concentrations at 0.01–0.5 mM resulted in cleavage and blastocyst rates similar to the controls for both PA and IVF embryos. Nicotine at 2.0 and 4.0 mM significantly decreased the cleavage rates and none of the embryos developed beyond the 16-cell stage. Nicotine might disrupt the polymerization of microfilaments leading to impaired chromosome alignment or segregation, and induce the formation of polynuclei with a variety of abnormal nuclear structures such as 2–6 nuclei, 2–4 metaphase plates, 2–4 sets of anaphase/telophase plates, and the co-existence of polynuclei and 2–4 sets of anaphase/telophase plates. Nicotine adversely affected blastocyst chromosomal composition. Fifty-six to 70% of the IVF blastocysts and 71–88% of the PA blastocysts were polyploid and/or mixoploid after culture in 0.2–1.0 mM nicotine-containing media, which were higher (P < 0.05 or P < 0.01) than the controls. Cell numbers of the nicotine-cultured blastocysts were significantly lower than the control. In conclusion, nicotine induced disfigured spindles and irregular chromosome alignment and possibly impaired cytokinesis, which lead to decreased quality of the yielded blastocysts. Mol. Reprod. Dev. 75: 801–809, 2008. © 2007 Wiley-Liss, Inc.