Expression and regulation of tumor necrosis factor (TNF) and TNF-receptor family members in the macaque corpus luteum during the menstrual cycle
Article first published online: 17 OCT 2008
Copyright © 2008 Wiley-Liss, Inc.
Molecular Reproduction and Development
Volume 76, Issue 4, pages 367–378, April 2009
How to Cite
Peluffo, M. C., Young, K. A., Hennebold, J. D. and Stouffer, R. L. (2009), Expression and regulation of tumor necrosis factor (TNF) and TNF-receptor family members in the macaque corpus luteum during the menstrual cycle. Mol. Reprod. Dev., 76: 367–378. doi: 10.1002/mrd.20970
- Issue published online: 20 FEB 2009
- Article first published online: 17 OCT 2008
- Manuscript Accepted: 13 AUG 2008
- Manuscript Received: 16 JUN 2008
Members of the tumor necrosis factor (TNF)-receptor (R) family may be involved in the tissue remodeling that occurs in the primate corpus luteum (CL) during development and regression. As a first step towards addressing this issue, studies assessed TNF ligand-R expression and regulation in CL collected from monkeys during the early (ECL, Days 3–5), mid (MCL, Days 7–8), mid-late (MLCL, Days 10–11), late (LCL, Days 14–16), and very late (VLCL, menses) luteal phase of the menstrual cycle. CL were also collected after gonadotropin and/or steroid ablation and replacement (with hLH and the progestin R5020) for 3 days at mid-late luteal phase. TNF-α, -β, FAS ligand (FASL), and TNF-R1 mRNA levels were two- to sixfold greater (P < 0.05) at the MLCL or LCL phase as compared to earlier (ECL, MCL). In contrast, TNF-R2 and FAS mRNA levels did not change during the luteal phase. Immunohistochemical staining for TNF-β, TNF-R1, TNF-R2, FAS, and FASL was observed in luteal cells, whereas only TNF-β staining was observed in endothelial cells. Several TNF-R components were influenced by LH and/or steroid ablation; notably, steroid ablation reduced (P < 0.05) luteal TNF-α, but not TNF-β, mRNA levels, which was prevented by progestin treatment. In contrast, steroid ablation increased (P < 0.05) luteal cell immunostaining for FAS and FASL, which was reduced by progestin treatment. Thus, several members of the TNF R-ligand family are expressed in the primate CL in an LH- and/or progestin-dependent manner. Peak expression in the late luteal phase may signify a role for the TNF-R system in death receptor-mediated apoptosis during luteolysis. Mol. Reprod. Dev. 76: 367–378, 2009. © 2008 Wiley-Liss, Inc.