Research Article

Prostasomal DNA characterization and transfer into human sperm

  1. Göran K. Ronquist1,
  2. Anders Larsson1,*,
  3. Gunnar Ronquist1,
  4. Anders Isaksson2,
  5. Julius Hreinsson3,
  6. Lena Carlsson1,
  7. Anneli Stavreus-Evers3

Article first published online: 2 JUN 2011

DOI: 10.1002/mrd.21327

Issue

Molecular Reproduction and Development

Molecular Reproduction and Development

Volume 78, Issue 7, pages 467–476, July 2011

Additional Information

How to Cite

Ronquist, G. K., Larsson, A., Ronquist, G., Isaksson, A., Hreinsson, J., Carlsson, L. and Stavreus-Evers, A. (2011), Prostasomal DNA characterization and transfer into human sperm. Mol. Reprod. Dev., 78: 467–476. doi: 10.1002/mrd.21327

Author Information

  1. 1

    Department of Medical Sciences, Clinical Chemistry, Uppsala University, Uppsala, Sweden

  2. 2

    Department of Medical Sciences, Cancer Pharmacology and Informatics, Uppsala University, Uppsala, Sweden

  3. 3

    Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden

*Department of Medical Sciences, Clinical Chemistry, Uppsala University, Uppsala 75185, Sweden.

Publication History

  1. Issue published online: 19 JUL 2011
  2. Article first published online: 2 JUN 2011
  3. Accepted manuscript online: 12 MAY 2011 12:04PM EST
  4. Manuscript Accepted: 2 MAY 2011
  5. Manuscript Received: 4 MAR 2011

Funded by

  • Uppsala University Hospital Research Fund (ALF). Grant Number: 2005-7293, 2007-4501

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Abstract

Human prostasomes, exosome-like microvesicles secreted by acinar cells of the prostate gland, contain chromosomal DNA. Agarose gel electrophoresis of DNA from seminal prostasomes displayed fragments of over 12 kb and smaller, with a distinct band around 1 kb that was excised, cloned, and sequenced. The sequences showed 8 out of 25 clones (32%) originating from genes. We elaborated the concept further by carrying out a genome-wide DNA copy number analysis of prostasomal DNA, hypothesizing that human prostasomes contain fragments of DNA randomly selected from the entire genome. Acridine orange-stained prostasomes were incubated with freshly prepared sperm for different times, and a transfer of acridine orange-stained prostasomal DNA to sperm (preferentially the head region) was observed. Fluorescence microscopy of slices in the center of 14 optical slides of the sperm head displayed an even fluorescence rather than a halo-like one, indicating DNA-uptake rather than just binding along the sperm head membrane. Mol. Reprod. Dev. 78:467–476, 2011. © 2011 Wiley-Liss, Inc.

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