Stereotaxic assembly and procedures for simultaneous electrophysiological and MRI study of conscious rat
Article first published online: 15 APR 2003
Copyright © 2003 Wiley-Liss, Inc.
Magnetic Resonance in Medicine
Volume 49, Issue 5, pages 962–967, May 2003
How to Cite
Khubchandani, M., Mallick, H.N., Jagannathan, N.R. and Mohan Kumar, V. (2003), Stereotaxic assembly and procedures for simultaneous electrophysiological and MRI study of conscious rat. Magn Reson Med, 49: 962–967. doi: 10.1002/mrm.10441
- Issue published online: 15 APR 2003
- Article first published online: 15 APR 2003
- Manuscript Revised: 23 DEC 2002
- Manuscript Accepted: 23 DEC 2002
- Manuscript Received: 21 FEB 2002
- Indian Council of Medical Research. Grant Number: 55/3/99-BMS II
- Council for Scientific and Industrial Research
- conscious animal;
A stereotaxic restraining assembly was designed and developed for simultaneous electrophysiological recordings and functional MRI (fMRI) data acquisition from a conscious rat. The design of the nonmagnetic stereotaxic apparatus facilitated the restraining of head and body of the unanesthetized conscious animal during MRI experiments. The apparatus was made of Teflon and Perspex materials with an appropriate size and shape for a 4.7 T / 40 cm animal MRI scanner. Electrodes made from nonmagnetic silver wire were implanted on the skull for recording the electroencephalogram (EEG), the electro-oculogram (EOG), and the electromyogram (EMG), while polycarbonate screws were used for anchoring the electrode assembly. There were no major distortions or artifacts observed in the electrophysiological tracings and MR images. Electrophysiological recordings during fMRI acquisitions are useful to study different neurophysiological mechanisms of sleep and pathophysiology of seizure activity. Integration of electrophysiological recordings (with their good temporal resolution) and MRI (with its superior spatial resolution) is helpful in characterizing the functional state of different brain regions. Magn Reson Med 49:962–967, 2003. © 2003 Wiley-Liss, Inc.