Rapid three-dimensional diffusion MRI facilitates the study of acute stroke in mice

Authors

  • Rong Xue,

    1. Department of Radiology, Division of MRI Research, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    2. Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    Search for more papers by this author
  • Masahiko Sawada,

    1. Department of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    Search for more papers by this author
  • Shozo Goto,

    1. Department of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    Search for more papers by this author
  • Patricia D. Hurn,

    1. Department of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    Search for more papers by this author
  • Richard J. Traystman,

    1. Department of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    Search for more papers by this author
  • Peter C.M. van Zijl,

    1. Department of Radiology, Division of MRI Research, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    Search for more papers by this author
  • Susumu Mori

    Corresponding author
    1. Department of Radiology, Division of MRI Research, School of Medicine, Johns Hopkins University, Baltimore, Maryland
    • Department of Radiology, School of Medicine, Johns Hopkins University, 217 Traylor Bldg., 720 Rutland Ave., Baltimore, MD 21205
    Search for more papers by this author

Abstract

MRI studies using mouse brain models of ischemia are becoming a valuable tool for understanding the mechanism of stroke, since transgenic models are now available. However, the small size of the mouse brain and the surgical complexity of creating ischemia in mice make it technically challenging to obtain high-quality MRI data. Therefore, there are few reports of MRI studies in murine cerebral ischemia. In this project a newly developed rapid 3D diffusion-weighted imaging (DWI) technique was applied to study experimental stroke in a mouse model of reversible middle cerebral artery occlusion (MCAO). Ischemic volumes were successfully delineated using this 3D whole-brain imaging technique with high spatial (0.34 × 0.5 × 1.0 mm3 before zero-filling) and temporal (7 min) resolution. The 3D observation revealed the characteristic evolution of stroke after transient MCAO. There was a temporarily high diffusion constant in the cortex during early reperfusion, followed by a secondary energy failure in the cortex and caudate-putamen at 6 and 21 h of reperfusion. Magn Reson Med 46:183–188, 2001. © 2001 Wiley-Liss, Inc.

Ancillary