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Glucose production pathways by 2H and 13C NMR in patients with HIV-associated lipoatrophy†

  1. Brian C. Weis1,2,3,
  2. David Margolis2,3,
  3. Shawn C. Burgess1,
  4. Matthew E. Merritt1,
  5. Holly Wise3,
  6. A. Dean Sherry1,4,
  7. Craig R. Malloy1,2,3,*

Article first published online: 26 MAR 2004

DOI: 10.1002/mrm.20057

Issue

Magnetic Resonance in Medicine

Magnetic Resonance in Medicine

Volume 51, Issue 4, pages 649–654, April 2004

Additional Information

How to Cite

Weis, B. C., Margolis, D., Burgess, S. C., Merritt, M. E., Wise, H., Sherry, A. D. and Malloy, C. R. (2004), Glucose production pathways by 2H and 13C NMR in patients with HIV-associated lipoatrophy. Magnetic Resonance in Medicine, 51: 649–654. doi: 10.1002/mrm.20057

Author Information

  1. 1

    The Mary Nell and Ralph B. Rogers Magnetic Resonance Center, Department of Radiology, University of Texas Southwestern Medical Center, Dallas, Texas

  2. 2

    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas

  3. 3

    VA North Texas Health Care System, Dallas, Texas

  4. 4

    Department of Chemistry, University of Texas at Dallas, Richardson, Texas

*Mary Nell and Ralph B. Rogers Magnetic Resonance Center, 5801 Forest Park Road, Dallas, TX 75235-9085

  1. †

    This article is a US Government work and, as such, is in the public domain in the United States of America.

Publication History

  1. Issue published online: 26 MAR 2004
  2. Article first published online: 26 MAR 2004
  3. Manuscript Accepted: 10 OCT 2003
  4. Manuscript Revised: 2 OCT 2003
  5. Manuscript Received: 13 AUG 2003

Funded by

  • National Center for Research Resources and the Clinical Research Unit of VA North Texas Health Care System. Grant Number: RR-02584

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Keywords:

  • glycerol;
  • gluconeogenesis;
  • deuterium;
  • lipodystrophy

Abstract

Patients with HIV taking protease inhibitors were selected for the presence (five subjects) or absence (five subjects) of lipoatrophy. Following an overnight fast, subjects were given oral 2H2O in divided doses (5 mL/kg body water), [U-13C3] propionate (10 mg/kg), and acetaminophen (1000 mg). Glucose (from plasma) or acetaminophen glucuronide (from urine) were converted to monoacetone glucose for 2H NMR and 13C NMR analysis. The fraction of plasma glucose derived from gluconeogenesis was not significantly different between groups. However, flux from glycerol into gluconeogenesis relative to glucose production was increased from 0.20 ± 0.13 among subjects without lipoatrophy to 0.42 ± 0.12 (P < 0.05) among subjects with lipoatrophy, and the TCA cycle contribution was reduced. Lipoatrophy was associated with an abnormal profile of glucose production as assessed by 13C and 2H NMR of plasma and urine. Magn Reson Med 51:649–654, 2004. Published 2004 Wiley-Liss, Inc.

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