This article is a US Government work and, as such, is in the public domain in the United States of America.
In vivo detection of cortical GABA turnover from intravenously infused [1-13C]D-glucose†
Article first published online: 19 MAY 2005
Published 2005 Wiley-Liss, Inc.
Magnetic Resonance in Medicine
Volume 53, Issue 6, pages 1258–1267, June 2005
How to Cite
Yang, J., Li, C. Q. and Shen, J. (2005), In vivo detection of cortical GABA turnover from intravenously infused [1-13C]D-glucose. Magn Reson Med, 53: 1258–1267. doi: 10.1002/mrm.20473
- Issue published online: 19 MAY 2005
- Article first published online: 19 MAY 2005
- Manuscript Accepted: 30 DEC 2004
- Manuscript Revised: 23 NOV 2004
- Manuscript Received: 5 OCT 2004
- GABA turnover;
- cerebral metabolism;
- high-field in vivo spectroscopy;
In this study [2-13C] γ-aminobutyric acid (GABA) was spectrally resolved in vivo and detected simultaneously with [4-13C]glutamate (Glu) and [4-13C]glutamine (Gln) in the proton spectra obtained from a localized 40 μL voxel in rat neocortex with the use of an adiabatic 1H-observed, 13C-edited (POCE) spectroscopy method and an 89-mm-bore vertical 11.7 Tesla microimager. The time-resolved kinetics of 13C label incorporation from intravenously infused [1-13C]glucose into [4-13C]Glu, [4-13C]Gln, and [2-13C]GABA were measured after acute administration of gabaculine, a potent and specific inhibitor of GABA-transaminase. In contrast to previous observations of a rapid turnover of [2-13C]GABA from [1-13C]glucose in intact rat brain, the rate of 13C incorporation from [1-13C]glucose into [2-13C]GABA in the gabaculine-treated rats was found to be significantly reduced as a result of the blockade of the GABA shunt. Magn Reson Med 53:1258–1267, 2005. Published 2005 Wiley-Liss, Inc.