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Conflicts of interest: The hyperpolarizer is on loan from GE Healthcare and is the subject of a research agreement between the University of Cambridge and GE Healthcare. GE supplied the trityl radical used in the hyperpolarization and Imaginia provided the glutamine formulation. The polarizer and related materials were provided by GE-Healthcare
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Communication
13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine†
Article first published online: 29 JUL 2008
DOI: 10.1002/mrm.21650
Copyright © 2008 Wiley-Liss, Inc.
Additional Information
How to Cite
Gallagher, F. A., Kettunen, M. I., Day, S. E., Lerche, M. and Brindle, K. M. (2008), 13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine. Magn Reson Med, 60: 253–257. doi: 10.1002/mrm.21650
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Publication History
- Issue published online: 29 JUL 2008
- Article first published online: 29 JUL 2008
- Manuscript Accepted: 12 MAR 2008
- Manuscript Revised: 19 FEB 2008
- Manuscript Received: 14 DEC 2007
Funded by
- Cancer Research UK (CRUK) & Royal College of Radiologists (UK) clinical research training fellowship. Grant Number: C19212/A6078
- NIH-Cambridge studentship
- CRUK program. Grant Number: CUK grant C197/A3514
Keywords:
- hyperpolarized 13C;
- dynamic nuclear polarization;
- glutamine metabolism;
- hepatocellular carcinoma
Abstract
Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity of 13C MR spectroscopy (MRS). [5-13C1]Glutamine was hyperpolarized using this technique by up to 5%, representing a 6000-fold increase in sensitivity. The conversion of hyperpolarized glutamine to glutamate by mitochondrial glutaminase was demonstrated using 13C-MRS measurements in cultured human hepatoma cells (HepG2). These results represent the first step in developing an imaging technique for detecting glutamine metabolism in vivo. Furthermore, since glutamine utilization has been correlated with cell proliferation, the study suggests a new technique for detecting changes in tumor cell proliferation. Magn Reson Med 60:253–257, 2008. © 2008 Wiley-Liss, Inc.