In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system

Authors

  • Pierre Smirnov,

    1. Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7 - Denis Diderot, Paris, France
    2. Laboratoire de Recherche en Imagerie, Université Paris Descartes, Faculté de Médecine Necker, Paris, France
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    • Drs. Smirnov and Poirier-Quinot contributed equally to this work

  • Marie Poirier-Quinot,

    1. Unité de Recherche en Résonance Magnétique Médicale, UMR 8081 CNRS, Université Paris-Sud -CNRS, Orsay, France
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    • Drs. Smirnov and Poirier-Quinot contributed equally to this work

  • Claire Wilhelm,

    1. Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7 - Denis Diderot, Paris, France
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  • Elise Lavergne,

    1. Laboratoire d'Immunologie Cellulaire, Inserm U543, Université Pierre et Marie Curie (UPMC), Faculté de Médecine, Paris, France
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  • Jean-Christophe Ginefri,

    1. Unité de Recherche en Résonance Magnétique Médicale, UMR 8081 CNRS, Université Paris-Sud -CNRS, Orsay, France
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  • Béhazine Combadière,

    1. Laboratoire d'Immunologie Cellulaire, Inserm U543, Université Pierre et Marie Curie (UPMC), Faculté de Médecine, Paris, France
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  • Olivier Clément,

    1. Laboratoire de Recherche en Imagerie, Université Paris Descartes, Faculté de Médecine Necker, Paris, France
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  • Luc Darrasse,

    1. Unité de Recherche en Résonance Magnétique Médicale, UMR 8081 CNRS, Université Paris-Sud -CNRS, Orsay, France
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  • Florence Gazeau

    Corresponding author
    1. Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7 - Denis Diderot, Paris, France
    • Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7 - Denis Diderot, Bâtiment Condorcet MSC - Case 7056, F-75205 Paris Cedex 13, France
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Abstract

We demonstrate the feasibility of detecting individual tumor-infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high-resolution surface coil. Using a recently developed high-temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 μm)3 at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin-expressing tumors by transferred OVA antigen-specific cytotoxic lymphocytes with low iron load. Magn Reson Med 60:1292–1297, 2008. © 2008 Wiley-Liss, Inc.

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