Communication

In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system

  1. Pierre Smirnov1,2,†,
  2. Marie Poirier-Quinot3,†,
  3. Claire Wilhelm1,
  4. Elise Lavergne4,
  5. Jean-Christophe Ginefri3,
  6. Béhazine Combadière4,
  7. Olivier Clément2,
  8. Luc Darrasse3,
  9. Florence Gazeau1,*

Article first published online: 24 NOV 2008

DOI: 10.1002/mrm.21812

Issue

Magnetic Resonance in Medicine

Magnetic Resonance in Medicine

Volume 60, Issue 6, pages 1292–1297, December 2008

Additional Information

How to Cite

Smirnov, P., Poirier-Quinot, M., Wilhelm, C., Lavergne, E., Ginefri, J.-C., Combadière, B., Clément, O., Darrasse, L. and Gazeau, F. (2008), In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system. Magn Reson Med, 60: 1292–1297. doi: 10.1002/mrm.21812

Author Information

  1. 1

    Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7 - Denis Diderot, Paris, France

  2. 2

    Laboratoire de Recherche en Imagerie, Université Paris Descartes, Faculté de Médecine Necker, Paris, France

  3. 3

    Unité de Recherche en Résonance Magnétique Médicale, UMR 8081 CNRS, Université Paris-Sud -CNRS, Orsay, France

  4. 4

    Laboratoire d'Immunologie Cellulaire, Inserm U543, Université Pierre et Marie Curie (UPMC), Faculté de Médecine, Paris, France

  1. †

    Drs. Smirnov and Poirier-Quinot contributed equally to this work

*Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7 - Denis Diderot, Bâtiment Condorcet MSC - Case 7056, F-75205 Paris Cedex 13, France

  1. †

    Drs. Smirnov and Poirier-Quinot contributed equally to this work

Publication History

  1. Issue published online: 24 NOV 2008
  2. Article first published online: 24 NOV 2008
  3. Manuscript Accepted: 13 AUG 2008
  4. Manuscript Revised: 9 JUL 2008
  5. Manuscript Received: 11 FEB 2008

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Keywords:

  • izon oxide nanoparticles;
  • cancer;
  • cell imaging;
  • high resolution MRI;
  • high-temperature superconducting coil

Abstract

We demonstrate the feasibility of detecting individual tumor-infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high-resolution surface coil. Using a recently developed high-temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 μm)3 at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin-expressing tumors by transferred OVA antigen-specific cytotoxic lymphocytes with low iron load. Magn Reson Med 60:1292–1297, 2008. © 2008 Wiley-Liss, Inc.

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