Mohanraja Kumar and Zdravka Medarova contributed equally.
Novel membrane-permeable contrast agent for brain tumor detection by MRI
Article first published online: 9 FEB 2010
Copyright © 2010 Wiley-Liss, Inc.
Magnetic Resonance in Medicine
Volume 63, Issue 3, pages 617–624, March 2010
How to Cite
Kumar, M., Medarova, Z., Pantazopoulos, P., Dai, G. and Moore, A. (2010), Novel membrane-permeable contrast agent for brain tumor detection by MRI. Magn Reson Med, 63: 617–624. doi: 10.1002/mrm.22216
- Issue published online: 25 FEB 2010
- Article first published online: 9 FEB 2010
- Manuscript Accepted: 28 AUG 2009
- Manuscript Revised: 21 AUG 2009
- Manuscript Received: 18 MAR 2009
- brain tumor;
- MR imaging;
- fluorescence imaging;
- magnetic nanoparticles;
- contrast agent
One of the key challenges hindering the clinical intervention against brain cancer is defined by the inability to detect brain tumors at an early enough stage to permit effective therapy. Furthermore, the rapid growth and severe lethality of this form of cancer predicate the vital importance of monitoring the development of the pathology and its outcome after therapeutic intervention. With this in mind, we designed a novel membrane-permeant contrast agent, MN-MPAP-Cy5.5, which consists of a superparamagnetic iron oxide core, for MRI conjugated to myristoylated polyarginine peptides, as a membrane translocation module and labeled with the near-infrared dye Cy5.5 for correlative microscopy. This probe showed a remarkable uptake by U-87 human glioma cells in vitro and localized and delineated stereotactically injected tumor in vivo by MRI. Our findings suggest that the agent mediates its effects by translocation of the magnetic nanoparticles label across the leaky tumor vasculature, followed by enhanced accumulation in tumor cells. The noninvasive detection of brain tumors when they are still small represents a formidable challenge from an imaging standpoint. Our study describes an improved strategy to detect brain lesions by utilizing a contrast agent with membrane translocation properties. Magn Reson Med, 2010. © 2010 Wiley-Liss, Inc.