Detecting response of rat C6 glioma tumors to radiotherapy using hyperpolarized [1-13C]pyruvate and 13C magnetic resonance spectroscopic imaging

Authors

  • Sam E. Day,

    1. Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA
    2. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
    Search for more papers by this author
  • Mikko I. Kettunen,

    1. Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge, United Kingdom
    2. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
    Search for more papers by this author
  • Murali Krishna Cherukuri,

    1. Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA
    Search for more papers by this author
  • James B. Mitchell,

    1. Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA
    Search for more papers by this author
  • Martin J. Lizak,

    1. Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA
    Search for more papers by this author
  • H. Douglas Morris,

    1. Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA
    Search for more papers by this author
  • Shingo Matsumoto,

    1. Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA
    Search for more papers by this author
  • Alan P. Koretsky,

    1. Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA
    Search for more papers by this author
  • Kevin M. Brindle

    Corresponding author
    1. Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge, United Kingdom
    2. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
    • Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Search for more papers by this author

Abstract

We show here that hyperpolarized [1-13C]pyruvate can be used to detect treatment response in a glioma tumor model; a tumor type where detection of response with 18fluoro-2-deoxyglucose, using positron emission tomography, is limited by the high background signals from normal brain tissue. 13C chemical shift images acquired following intravenous injection of hyperpolarized [1-13C]pyruvate into rats with implanted C6 gliomas showed significant labeling of lactate within the tumors but comparatively low levels in surrounding brain.Labeled pyruvate was observed at high levels in blood vessels above the brain and from other major vessels elsewhere but was detected at only low levels in tumor and brain.The ratio of hyperpolarized 13C label in tumor lactate compared to the maximum pyruvate signal in the blood vessels was decreased from 0.38 ± 0.16 to 0.23 ± 0.13, (a reduction of 34%) by 72 h following whole brain irradiation with 15 Gy. Magn Reson Med, 2011. © 2010 Wiley-Liss, Inc.

Ancillary