Detecting response of rat C6 glioma tumors to radiotherapy using hyperpolarized [1-13C]pyruvate and 13C magnetic resonance spectroscopic imaging
Article first published online: 16 NOV 2010
Copyright © 2010 Wiley-Liss, Inc.
Magnetic Resonance in Medicine
Volume 65, Issue 2, pages 557–563, February 2011
How to Cite
Day, S. E., Kettunen, M. I., Cherukuri, M. K., Mitchell, J. B., Lizak, M. J., Morris, H. D., Matsumoto, S., Koretsky, A. P. and Brindle, K. M. (2011), Detecting response of rat C6 glioma tumors to radiotherapy using hyperpolarized [1-13C]pyruvate and 13C magnetic resonance spectroscopic imaging. Magn Reson Med, 65: 557–563. doi: 10.1002/mrm.22698
- Issue published online: 24 JAN 2011
- Article first published online: 16 NOV 2010
- Manuscript Accepted: 3 OCT 2010
- Manuscript Revised: 4 SEP 2010
- Manuscript Received: 9 JUL 2010
- Cancer Research UK Programme grant. Grant Number: C197/A3514
- NCI (Intramural Research Program)
We show here that hyperpolarized [1-13C]pyruvate can be used to detect treatment response in a glioma tumor model; a tumor type where detection of response with 18fluoro-2-deoxyglucose, using positron emission tomography, is limited by the high background signals from normal brain tissue. 13C chemical shift images acquired following intravenous injection of hyperpolarized [1-13C]pyruvate into rats with implanted C6 gliomas showed significant labeling of lactate within the tumors but comparatively low levels in surrounding brain.Labeled pyruvate was observed at high levels in blood vessels above the brain and from other major vessels elsewhere but was detected at only low levels in tumor and brain.The ratio of hyperpolarized 13C label in tumor lactate compared to the maximum pyruvate signal in the blood vessels was decreased from 0.38 ± 0.16 to 0.23 ± 0.13, (a reduction of 34%) by 72 h following whole brain irradiation with 15 Gy. Magn Reson Med, 2011. © 2010 Wiley-Liss, Inc.