Absolute cerebral blood flow quantification with pulsed arterial spin labeling during hyperoxia corrected with the simultaneous measurement of the longitudinal relaxation time of arterial blood

Authors

  • David T. Pilkinton,

    Corresponding author
    1. Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    2. Center for Magnetic Resonance and Optical Imaging (CMROI), Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    • University of Pennsylvania, B1 Stellar Chance Labs, 422 Curie Boulevard, Philadelphia, PA 19104
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  • Teruyuki Hiraki,

    1. Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
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  • John A. Detre,

    1. Center for Magnetic Resonance and Optical Imaging (CMROI), Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    2. Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    3. Center for Function Neuroimaging, Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
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  • Joel H. Greenberg,

    1. Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
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  • Ravinder Reddy

    1. Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    2. Center for Magnetic Resonance and Optical Imaging (CMROI), Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
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Abstract

Quantitative arterial spin labeling (ASL) estimates of cerebral blood flow (CBF) during oxygen inhalation are important in several contexts, including functional experiments calibrated with hyperoxia and studies investigating the effect of hyperoxia on regional CBF. However, ASL measurements of CBF during hyperoxia are confounded by the reduction in the longitudinal relaxation time of arterial blood (T1a) from paramagnetic molecular oxygen dissolved in blood plasma. The aim of this study is to accurately quantify the effect of arbitrary levels of hyperoxia on T1a and correct ASL measurements of CBF during hyperoxia on a per-subject basis. To mitigate artifacts, including the inflow of fresh spins, partial voluming, pulsatility, and motion, a pulsed ASL approach was implemented for in vivo measurements of T1a in the rat brain at 3 Tesla. After accounting for the effect of deoxyhemoglobin dilution, the relaxivity of oxygen on blood was found to closely match phantom measurements. The results of this study suggest that the measured ASL signal changes are dominated by reductions in T1a for brief hyperoxic inhalation epochs, while the physiologic effects of oxygen on the vasculature account for most of the measured reduction in CBF for longer hyperoxic exposures. Magn Reson Med, 2011. © 2011 Wiley-Liss, Inc.

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