Reut Avni and Tal Raz contributed equally to this work.
Unique in utero identification of fetuses in multifetal mouse pregnancies by placental bidirectional arterial spin labeling MRI
Article first published online: 8 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 68, Issue 2, pages 560–570, August 2012
How to Cite
Avni, R., Raz, T., Biton, I. E., Kalchenko, V., Garbow, J. R. and Neeman, M. (2012), Unique in utero identification of fetuses in multifetal mouse pregnancies by placental bidirectional arterial spin labeling MRI. Magn Reson Med, 68: 560–570. doi: 10.1002/mrm.23246
- Issue published online: 12 JUL 2012
- Article first published online: 8 DEC 2011
- Manuscript Accepted: 12 SEP 2011
- Manuscript Revised: 10 AUG 2011
- Manuscript Received: 14 JUN 2011
- 7th Framework European Research Council Advanced grant. Grant Number: 232640-IMAGO
- Helen and Morris Mauerberger Chair in Biological Sciences
- in vivo imaging
Noninvasive imaging is a critical part of the study of developing embryos/fetuses, particularly in the context of alterations of gene expression in genetically modified animals. However, in litter-bearing animals, such as mice, the inability to accurately identify individual embryo/fetus in utero is a major obstacle to longitudinal, noninvasive in vivo studies. Arterial spin labeling MRI was adopted here to determine the fetal order along the uterine horns in vivo, based on the specific pattern of dual arterial blood supply within the mouse uterine horns. Blood enters the mouse uterus cranially through the ovarian artery and caudally through the uterine artery. Saturation slices were alternately placed on the maternal heart or on the bifurcation point of the common iliac artery, thereby saturating either downward inflow via the ovarian arteries or upward inflow via the uterine arteries, respectively. Saturation maps provided a unique signature with highly significant correlation between the direction-dependent magnetization transfer and the position of the fetuses/placentas along the uterine horns. The bidirectional arterial spin labeling-MRI method reported here opens possibilities to determine and pursue phenotypic alterations in fetuses and placentas in longitudinal studies of transgenic and knockout mice models, and for studying defects in placental vascular architecture. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc.