The pore size of PLGA bone implants determines the de novo formation of bone tissue in tibial head defects in rats
Version of Record online: 19 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 70, Issue 4, pages 925–935, October 2013
How to Cite
Penk, A., Förster, Y., Scheidt, H. A., Nimptsch, A., Hacker, M. C., Schulz-Siegmund, M., Ahnert, P., Schiller, J., Rammelt, S. and Huster, D. (2013), The pore size of PLGA bone implants determines the de novo formation of bone tissue in tibial head defects in rats. Magn Reson Med, 70: 925–935. doi: 10.1002/mrm.24541
- Issue online: 24 SEP 2013
- Version of Record online: 19 NOV 2012
- Manuscript Revised: 4 OCT 2012
- Manuscript Accepted: 4 OCT 2012
- Manuscript Received: 31 JUL 2012
- DFG. Grant Number: TRR 67, projects: A1, A2, A6, B5
- magic-angle spinning NMR;
- order parameters;
- bone collagen;
- bone apatite
The influence of the pore size of biodegradable poly(lactic-co-glycolic acid) scaffolds on bone regeneration was investigated.
Cylindrical poly(lactic-co-glycolic acid) scaffolds were implanted into a defect in the tibial head of rats. Pore sizes of 100–300, 300–500, and 500–710 μm were tested and compared to untreated defects as control. Two and four weeks after implantation, the specimens were explanted and defect regeneration and de novo extracellular matrix generation were investigated by MRI, quantitative solid-state NMR, and mass spectrometry.
The pore size of the scaffolds had a pronounced influence on the quantity of the extracellular matrix synthesized in the graft; most collagen was synthesized within the first 2 weeks of implantation, while the amount of hydroxyapatite increased in the second 2 weeks. After 4 weeks, the scaffolds contained large quantities of newly formed lamellar bone while the control defects were filled by inhomogenous woven bone. Best results were obtained for scaffolds of a pore size of 300–500 μm.
Our analysis showed that the structure and dynamics of the regenerated extracellular matrix was very similar to that of the native bone, suggesting that biomineralization was significantly enhanced by the choice of the most appropriate implant material. Magn Reson Med, 70:925–935, 2013. © 2012 Wiley Periodicals, Inc.