Enhanced tissue differentiation in the developing mouse brain using magnetic resonance micro-histology
Version of Record online: 4 DEC 2012
Copyright © 2012 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 70, Issue 5, pages 1380–1388, November 2013
How to Cite
Norris, F. C., Betts-Henderson, J., Wells, J. A., Cleary, J. O., Siow, B. M., Walker-Samuel, S., McCue, K., Salomoni, P., Scambler, P. J. and Lythgoe, M. F. (2013), Enhanced tissue differentiation in the developing mouse brain using magnetic resonance micro-histology. Magn Reson Med, 70: 1380–1388. doi: 10.1002/mrm.24573
- Issue online: 25 OCT 2013
- Version of Record online: 4 DEC 2012
- Manuscript Accepted: 30 OCT 2012
- Manuscript Revised: 29 OCT 2012
- Manuscript Received: 14 JUL 2012
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council
- British Heart Foundation
- microscopic magnetic resonance imaging;
- mouse embryo phenotyping;
- tissue-specific contrast enhancement;
- embryonic cellular density;
- neural stem cell delineation;
- magnetic resonance histology
Purpose: Worldwide efforts to understand developmental processes demand new high-resolution 3D imaging methods to detect the consequences of gene function in embryo development and diseases. Encouragingly, recent studies have shown that MRI contrast agents can highlight specific tissue structures in ex vivo adult mouse brains. MR imaging of mouse embryos is currently limited by a lack of tissue staining capabilities that would provide the flexibility and specificity offered by histological stains conventionally used for mouse embryo phenotyping.
Methods: The MRI staining properties of two readily available contrast agents, Mn-DPDP and Gd-DTPA, were investigated in mid-gestation mouse embryos.
Results: Brain tissue substructures not normally visible using MRI were detected. Mn-DPDP and Gd-DTPA provided spatially distinct tissue staining patterns. An initial assessment indicated that these agents utilized independent contrast enhancement mechanisms. Mn-DPDP was identified as a potential MRI contrast agent for enhancement of mouse embryonic cellular density and enabled identification of regions containing populations of neural stem and progenitor cells within the intact embryo brain.
Conclusions: Different contrast agents may be used to provide tissue-specific contrast enhancement, suggesting that a host of specialized MRI stains may be available for probing the developing mouse brain and investigating developmental and disease mechanisms. Magn Reson Med 70:1380–1388, 2013. © 2012 Wiley Periodicals, Inc.