Quantitative measurement of cancer metabolism using stimulated echo hyperpolarized carbon-13 MRS
Article first published online: 14 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 71, Issue 1, pages 1–11, January 2014
How to Cite
Swisher, C. L., Larson, P. E. Z., Kruttwig, K., Kerr, A. B., Hu, S., Bok, R. A., Goga, A., Pauly, J. M., Nelson, S. J., Kurhanewicz, J. and Vigneron, D. B. (2014), Quantitative measurement of cancer metabolism using stimulated echo hyperpolarized carbon-13 MRS. Magn Reson Med, 71: 1–11. doi: 10.1002/mrm.24634
- Issue published online: 17 DEC 2013
- Article first published online: 14 FEB 2013
- Manuscript Accepted: 19 DEC 2012
- Manuscript Revised: 28 NOV 2012
- Manuscript Received: 5 SEP 2012
- NIH . Grant Number: P41-EB013598
- National Science Foundation Graduate Research Fellowship
- NIGMS-IMSD . Grant Number: R25-GM56847
- NIH . Grant Number: R00 EB012064
- UCSF Liver Center . Grant Number: P30 DK026743
- hyperpolarized Carbon-13 MRS;
- stimulated echo;
Magnetic resonance spectroscopy of hyperpolarized substrates allows for the observation of label exchange catalyzed by enzymes providing a powerful tool to investigate tissue metabolism and potentially kinetics in vivo. However, the accuracy of current methods to calculate kinetic parameters has been limited by T1 relaxation effects, extracellular signal contributions, and reduced precision at lower signal-to-noise ratio.
Theory and Methods
To address these challenges, we investigated a new modeling technique using metabolic activity decomposition-stimulated echo acquisition mode. The metabolic activity decomposition-stimulated echo acquisition mode technique separates exchanging from nonexchanging metabolites providing twice the information as conventional techniques.
This allowed for accurate measurements of rates of conversion and of multiple T1 values simultaneously using a single acquisition.
The additional measurement of T1 values for the reaction metabolites provides further biological information about the cellular environment of the metabolites. The new technique was investigated through simulations and in vivo studies of transgenic mouse models of cancer demonstrating improved assessments of kinetic rate constants and new T1 relaxation value measurements for hyperpolarized 13C-pyruvate, 13C-lactate, and 13C-alanine. Magn Reson Med 71:1–11, 2014. © 2013 Wiley Periodicals, Inc.