Sensitivity and Source of Amine-Proton Exchange and Amide-Proton Transfer Magnetic Resonance Imaging in Cerebral Ischemia
Article first published online: 11 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 71, Issue 1, pages 118–132, January 2014
How to Cite
Zong, X., Wang, P., Kim, S.-G. and Jin, T. (2014), Sensitivity and Source of Amine-Proton Exchange and Amide-Proton Transfer Magnetic Resonance Imaging in Cerebral Ischemia. Magn Reson Med, 71: 118–132. doi: 10.1002/mrm.24639
- Issue published online: 17 DEC 2013
- Article first published online: 11 FEB 2013
- Manuscript Revised: 19 DEC 2012
- Manuscript Accepted: 19 DEC 2012
- Manuscript Received: 18 SEP 2012
- NIH. Grant Numbers: EB008717, EB003324, EB003375, NS44589
- cerebral ischemia;
- apparent diffusion coefficient;
- chemical exchange saturation transfer;
- spin locking;
- MR spectroscopy
Amide-proton transfer (APT) and amine–water proton exchange (APEX) MRI can be viable to map pH-decreasing ischemic regions. However, their exact contributions are unclear.
We measured APEX- and APT-weighted magnetization transfer ratio asymmetry (denoted as APEXw and APTw), apparent diffusion coefficient, T2, and T1 images and localized proton spectra in rats with permanent middle cerebral artery occlusion at 9.4 T. Phantoms and theoretical studies were also performed.
Within 1-h postocclusion, APEXw and APTw maps showed hyperintensity (3.1% of M0) and hypointensity (−1.8%), respectively, in regions with decreased apparent diffusion coefficient. Ischemia increased lactate and gamma aminobutyric acid concentrations, but decreased glutamate and taurine concentrations. Over time, the APEXw contrast decreased with glutamate, taurine, and creatine, whereas the APTw contrast and lactate level were similar. Phantom and theoretical studies suggest that the source of APEXw signal is mainly from proteins at normal pH, whereas at decreased pH, gamma aminobutyric acid and glutamate contributions increase, inducing the positive APEXw contrast in ischemic regions. The APTw contrast is sensitive to lactate concentration and pH, but contaminated from contributions of the faster APEX processes.
Positive APEXw contrast is more sensitive to ischemia than negative APTw contrast. They may provide complementary tissue metabolic information. Magn Reson Med 71:118–132, 2014. © 2013 Wiley Periodicals, Inc.