Ex vivo MR volumetry of human brain hemispheres
Article first published online: 25 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 71, Issue 1, pages 364–374, January 2014
How to Cite
Kotrotsou, A., Bennett, D. A., Schneider, J. A., Dawe, R. J., Golak, T., Leurgans, S. E., Yu, L. and Arfanakis, K. (2014), Ex vivo MR volumetry of human brain hemispheres. Magn Reson Med, 71: 364–374. doi: 10.1002/mrm.24661
- Issue published online: 17 DEC 2013
- Article first published online: 25 FEB 2013
- Manuscript Accepted: 7 JAN 2013
- Manuscript Revised: 21 DEC 2012
- Manuscript Received: 4 JUN 2012
- National Institute on Aging . Grant Numbers: P30AG10161 , R01AG15819 , R01AG17917
- Illinois Department of Public Health and the Chicago Chapter of the Achievements Reward for College Scientists Foundation
- ex vivo;
The aims of this work were to (a) develop an approach for ex vivo MR volumetry of human brain hemispheres that does not contaminate the results of histopathological examination, (b) longitudinally assess regional brain volumes postmortem, and (c) investigate the relationship between MR volumetric measurements performed in vivo and ex vivo.
An approach for ex vivo MR volumetry of human brain hemispheres was developed. Five hemispheres from elderly subjects were imaged ex vivo longitudinally. All datasets were segmented. The longitudinal behavior of volumes measured ex vivo was assessed. The relationship between in vivo and ex vivo volumetric measurements was investigated in seven elderly subjects imaged both antemortem and postmortem.
This approach for ex vivo MR volumetry did not contaminate the results of histopathological examination. For a period of 6 months postmortem, within-subject volume variation across time points was substantially smaller than intersubject volume variation. A close linear correspondence was detected between in vivo and ex vivo volumetric measurements.
Regional brain volumes measured with this approach for ex vivo MR volumetry remain relatively unchanged for a period of 6 months postmortem. Furthermore, the linear relationship between in vivo and ex vivo MR volumetric measurements suggests that this approach captures information linked to antemortem macrostructural brain characteristics. Magn Reson Med 71:364–374, 2014. © 2013 Wiley Periodicals, Inc.