Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle
Article first published online: 4 SEP 2013
Copyright © 2013 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 72, Issue 1, pages 8–19, July 2014
How to Cite
Triplett, W. T., Baligand, C., Forbes, S. C., Willcocks, R. J., Lott, D. J., DeVos, S., Pollaro, J., Rooney, W. D., Sweeney, H. L., Bönnemann, C. G., Wang, D.-J., Vandenborne, K. and Walter, G. A. (2014), Chemical shift-based MRI to measure fat fractions in dystrophic skeletal muscle. Magn Reson Med, 72: 8–19. doi: 10.1002/mrm.24917
- Issue published online: 16 JUN 2014
- Article first published online: 4 SEP 2013
- Manuscript Accepted: 15 JUL 2013
- Manuscript Revised: 27 JUN 2013
- Manuscript Received: 8 FEB 2013
- National Institute of Arthritis and Musculoskeletal and Skin Diseases/National Institute of Neurological Disorders and Stroke. Grant Number: R01AR056973
- National Institutes of Health; Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Centers. Grant Number: U54AR052646
- National Institutes of Health. Grant Number: P01HL59412
- Parent Project Muscular Dystrophy. A portion of this work was performed at the National High Magnetic Field Laboratory, which is supported by National Science Foundation Cooperative Agreement No. DMR-0654118 and the State of Florida
- Duchenne muscular dystrophy;
- collagen VI;
- skeletal muscle;
- magnetic resonance spectroscopy;
- congenital muscular dystrophy;
- muscle composition;
- fat water imaging
The relationship between fat fractions (FFs) determined based on multiple TE, unipolar gradient echo images and 1H magnetic resonance spectroscopy (MRS) was evaluated using different models for fat-water decomposition, signal-to-noise ratios, and excitation flip angles.
A combination of single-voxel proton spectroscopy (1H-MRS) and gradient echo imaging was used to determine muscle FFs in both normal and dystrophic muscles. In order to cover a large range of FFs, the soleus and vastus lateralis muscles of 22 unaffected control subjects, 16 subjects with collagen VI deficiency (COL6), and 71 subjects with Duchenne muscular dystrophy (DMD) were studied. 1H-MRS–based FF were corrected for the increased muscle 1H2O T1 and T2 values observed in dystrophic muscles.
Excellent agreement was found between coregistered FFs derived from gradient echo images fit to a multipeak model with noise bias correction and the relaxation-corrected 1H-MRS FFs (y = 0.93x + 0.003; R2 = 0.96) across the full range of FFs. Relaxation-corrected 1H-MRS FFs and imaging-based FFs were significantly elevated (P < 0.01) in the muscles of COL6 and DMD subjects.
FFs, T2, and T1 were all sensitive to muscle involvement in dystrophic muscle. MRI offered an additional advantage over single-voxel spectroscopy in that the tissue heterogeneity in FFs could be readily determined. Magn Reson Med 72:8–19, 2014. © 2013 Wiley Periodicals, Inc.