Noninvasive imaging of infection after treatment with tumor-homing bacteria using Chemical Exchange Saturation Transfer (CEST) MRI
Article first published online: 7 OCT 2013
Copyright © 2013 Wiley Periodicals, Inc.
Magnetic Resonance in Medicine
Volume 70, Issue 6, pages 1690–1698, December 2013
How to Cite
Liu, G., Bettegowda, C., Qiao, Y., Staedtke, V., Chan, K. W.Y., Bai, R., Li, Y., Riggins, G. J., Kinzler, K. W., Bulte, J. W.M., McMahon, M. T., Gilad, A. A., Vogelstein, B., Zhou, S. and van Zijl, P. C.M. (2013), Noninvasive imaging of infection after treatment with tumor-homing bacteria using Chemical Exchange Saturation Transfer (CEST) MRI. Magn Reson Med, 70: 1690–1698. doi: 10.1002/mrm.24955
- Issue published online: 18 NOV 2013
- Article first published online: 7 OCT 2013
- Manuscript Accepted: 22 AUG 2013
- Manuscript Revised: 12 AUG 2013
- Manuscript Received: 24 JUN 2013
- NIH. Grant Numbers: R01EB015032, R01EB012590, R21EB015609, R21EB008769, R21EB005252, P50 CA062924
- by BioMed Valley Discoveries, Inc.
- the Virginia and D.K. Ludwig Fund for Cancer Research
Additional Supporting Information may be found in the online version of this article.
Figure S1. In vitro CEST detection of C. novyi-NT. a) z-spectra of C. novyi-NT solution (3x107/mL), spore solution and medium (PBS+ 2% oxyrase), b) concentration dependency of CEST signal at 2.6ppm using three concentrations, and c) MTRasym maps at 2.6 ppm for bacteria in PBS at three concentration as marked (most left ), medium (second on right) and spore in PBS (most right, ∼3x107/mL).
Figure S2. In vitro CEST detection of C. novyi-NT in cell culture medium as shown by MTRasym plots for the four concentration samples (1.3–13 x106 cells/mL) and medium only. The acquisition condition is as same as that described in the Methods section, namely saturation power = 3.6µT, tsat= 3 sec. The temperature was kept constantly at 37°C.
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