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Two-site reproducibility of cerebellar and brainstem neurochemical profiles with short-echo, single-voxel MRS at 3T

Authors

  • Dinesh K. Deelchand,

    Corresponding author
    1. Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, Minnesota, USA
    • Correspondence to: Dinesh K. Deelchand, Ph.D., Center for Magnetic Resonance Research, University of Minnesota, 2021 6th St SE, Minneapolis, MN 55455, USA. E-mail: dinesh@cmrr.umn.edu

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  • Isaac M. Adanyeguh,

    1. INSERM UMR S975, Brain and Spine Institute, Hospital La Salpêtrière, Paris, France
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  • Uzay E. Emir,

    1. Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, Minnesota, USA
    Current affiliation:
    1. Oxford Centre for Functional MRI of the Brain, John Radcliffe Hospital, University of Oxford, Headington, Oxford, UK
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  • Tra-My Nguyen,

    1. INSERM UMR S975, Brain and Spine Institute, Hospital La Salpêtrière, Paris, France
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  • Romain Valabregue,

    1. INSERM UMR S975, Brain and Spine Institute, Hospital La Salpêtrière, Paris, France
    2. Institut du Cerveau et de la Moelle, Centre de NeuroImagerie de Recherche, Hôpital La Salpêtrière, Paris, France
    3. University Pierre and Marie Curie, Paris, France
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  • Pierre-Gilles Henry,

    1. Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, Minnesota, USA
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  • Fanny Mochel,

    1. INSERM UMR S975, Brain and Spine Institute, Hospital La Salpêtrière, Paris, France
    2. University Pierre and Marie Curie, Paris, France
    3. AP-HP, Department of Genetic, Hospital La Salpêtrière, Paris, France
    4. Neurometabolic Unit, Hospital La Salpêtrière, Paris, France
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  • Gülin Öz

    1. Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, Minnesota, USA
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Abstract

Purpose

To determine whether neurochemical concentrations obtained at two MRI sites using clinical 3T scanners can be pooled when a highly optimized, nonvendor short-echo, single-voxel proton MRS pulse sequence is used in conjunction with identical calibration and quantification procedures.

Methods

A modified semi-LASER sequence (TE = 28 ms) was used to acquire spectra from two brain regions (cerebellar vermis and pons) on two Siemens 3T scanners using the same B0 and B1 calibration protocols from two different cohorts of healthy volunteers (N = 24–33 per site) matched for age and body mass index. Spectra were quantified with LCModel using water scaling.

Results

The spectral quality was very consistent between the two sites and allowed reliable quantification of at least 13 metabolites in the vermis and pons compared with 3–5 metabolites in prior multisite magnetic resonance spectroscopy trials using vendor-provided sequences. The neurochemical profiles were nearly identical at the two sites and showed the feasibility to detect interindividual differences in the healthy brain.

Conclusion

Highly reproducible neurochemical profiles can be obtained on different clinical 3T scanners at different sites, provided that the same, optimized acquisition and analysis techniques are used. This will allow pooling of multisite data in clinical studies, which is particularly critical for rare neurological diseases. Magn Reson Med 73:1718–1725, 2015. © 2014 Wiley Periodicals, Inc.

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