Characterization of perfluorocarbon relaxation times and their influence on the optimization of fluorine-19 MRI at 3 tesla

Authors

  • Roberto Colotti,

    1. Department of Radiology, University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland
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  • Jessica A. M. Bastiaansen,

    1. Department of Radiology, University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland
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  • Anne Wilson,

    1. Ludwig Center for Cancer Research, University of Lausanne (UNIL), Epalinges, Switzerland
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  • Ulrich Flögel,

    1. Department of Cardiovascular Physiology, Heinrich Heine University, Düsseldorf, Germany
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  • Christine Gonzales,

    1. Division of Cardiology and Cardiac MR Center, Department of Internal Medicine, University Hospital of Lausanne (CHUV), Lausanne, Switzerland
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  • Juerg Schwitter,

    1. Division of Cardiology and Cardiac MR Center, Department of Internal Medicine, University Hospital of Lausanne (CHUV), Lausanne, Switzerland
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  • Matthias Stuber,

    1. Department of Radiology, University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland
    2. Center for Biomedical Imaging (CIBM), Lausanne and Geneva, Switzerland
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  • Ruud B. van Heeswijk

    Corresponding author
    1. Department of Radiology, University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland
    • Correspondence to: Ruud B. van Heeswijk, PhD, CardioVascular MR Center, Centre Hospitalier Universitaire Vaudois (CHUV), Rue de Bugnon 46, BH 8.84, 1011 Lausanne, Switzerland. E-mail: ruud.mri@gmail.com

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Abstract

Purpose

To characterize and optimize 19F MRI for different perfluorocarbons (PFCs) at 3T and quantify the loss of acquisition efficiency as a function of different temperature and cellular conditions.

Methods

The T1 and T2 relaxation times of the commonly used PFCs perfluoropolyether (PFPE), perfluoro-15-crown-5-ether (PFCE), and perfluorooctyl bromide (PFOB) were measured in phantoms and in several different conditions (cell types, presence of fixation agent, and temperatures). These relaxation times were used to optimize pulse sequences through numerical simulations. The acquisition efficiency in each cellular condition was then determined as the ratio of the signal after optimization with the reference relaxation times and after optimization with its proper relaxation times. Finally, PFC detection limits were determined.

Results

The loss of acquisition efficiency due to parameter settings optimized for the wrong temperature and cellular condition was limited to 13%. The detection limits of all PFCs were lower at 24 °C than at 37 °C and varied from 11.8 ± 3.0 mM for PFCE at 24 °C to 379.9 ± 51.8 mM for PFOB at 37 °C.

Conclusion

Optimizing 19F pulse sequences with a known phantom only leads to moderate loss in acquisition efficiency in cellular conditions that might be encountered in in vivo and in vitro experiments. Magn Reson Med, 2016. © 2016 Wiley Periodicals, Inc.

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