Transplantation of adult myoblasts or adipose tissue precursor cells by high-density injection failed to improve reinnervated skeletal muscles

Authors

  • Matthieu César MD,

    1. Institut National de la Recherche Agronomique, UMR 866 Différenciation Cellulaire et Croissance, 2 Place Pierre Viala, F-34060 Montpellier, France
    2. Service de Chirurgie Orthopédique 2 et Chirurgie de la Main, Centre Hospitalier Universitaire Lapeyronie, Montpellier, France
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  • Sophie Roussanne-Domergue MD,

    1. Institut National de la Recherche Agronomique, UMR 866 Différenciation Cellulaire et Croissance, 2 Place Pierre Viala, F-34060 Montpellier, France
    2. Service de Chirurgie Maxillo-Faciale et Chirurgie Plastique, Centre Hospitalier Universitaire Lapeyronie, Montpellier, France
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  • Bertrand Coulet MD, PhD,

    1. Service de Chirurgie Orthopédique 2 et Chirurgie de la Main, Centre Hospitalier Universitaire Lapeyronie, Montpellier, France
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  • Stéphanie Gay BSc,

    1. Institut National de la Recherche Agronomique, UMR 866 Différenciation Cellulaire et Croissance, 2 Place Pierre Viala, F-34060 Montpellier, France
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  • Jean-Paul Micallef DSc,

    1. Institut National de la Santé et de la Recherche Médicale, ADR 08, Montpellier, France
    2. UFR STAPS, EA 2991 Efficience et Déficience Motrices, Montpellier, France
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  • Michel Chammas MD, PhD,

    1. Service de Chirurgie Orthopédique 2 et Chirurgie de la Main, Centre Hospitalier Universitaire Lapeyronie, Montpellier, France
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  • Yves Reyne PhD,

    1. Institut National de la Recherche Agronomique, UMR 866 Différenciation Cellulaire et Croissance, 2 Place Pierre Viala, F-34060 Montpellier, France
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  • Francis Bacou DSc

    Corresponding author
    1. Institut National de la Recherche Agronomique, UMR 866 Différenciation Cellulaire et Croissance, 2 Place Pierre Viala, F-34060 Montpellier, France
    • Institut National de la Recherche Agronomique, UMR 866 Différenciation Cellulaire et Croissance, 2 Place Pierre Viala, F-34060 Montpellier, France
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Abstract

We previously showed that transfer of adult myoblasts (MB) into cardiotoxin-damaged muscle improved the properties of reinnervated tibialis anterior muscle of rabbits. However, this cell therapy protocol cannot be applied to humans because of the hazardous effects of the myotoxin. To circumvent this approach, we used the recently developed high-density injection technique to autotransplant cultured cells 1 mm from each other into the tibialis anterior muscle without previous cardiotoxin-induced damage. Two months after transection and immediate suture of the common peroneal nerve, we transferred by this technique two types of precursor cells, MB or cells isolated from the adipose tissue stromal vascular fraction. In contrast to our previous results, muscles studied at 4 months showed no benefits in terms of function or morphology, whatever the transferred cells. These results, together with the results of earlier studies, emphasize the importance of delivery methods and the muscle environment in supporting cell integration into host tissues. Muscle Nerve, 2007

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