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Effects of exercise and muscle type on BDNF, NT-4/5, and TrKB expression in skeletal muscle

Authors

  • Daniel I. Ogborn MSc,

    1. Health, Leisure and Human Performance Research Institute, Faculty of Kinesiology and Recreation Management, and Spinal Cord Research Center, University of Manitoba, 404 Basic Medical Sciences, 730 William Avenue, Winnipeg, Manitoba R3E 3J7, Canada
    Current affiliation:
    1. Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada
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  • Phillip F. Gardiner PhD

    Corresponding author
    1. Health, Leisure and Human Performance Research Institute, Faculty of Kinesiology and Recreation Management, and Spinal Cord Research Center, University of Manitoba, 404 Basic Medical Sciences, 730 William Avenue, Winnipeg, Manitoba R3E 3J7, Canada
    • Health, Leisure and Human Performance Research Institute, Faculty of Kinesiology and Recreation Management, and Spinal Cord Research Center, University of Manitoba, 404 Basic Medical Sciences, 730 William Avenue, Winnipeg, Manitoba R3E 3J7, Canada
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Abstract

Muscle-derived neurotrophins are thought to contribute to the adaptation of skeletal muscle to exercise, but the effects of brief exercise interventions on BDNF, NT-4/5, and trkB are not understood. RNA was extracted for RT-PCR from soleus and medial gastrocnemius of Sprague-Dawley rats exercised on a treadmill at speeds up to 20 m/min at 5% incline for 5 or 10 days. BDNF expression was elevated in soleus following 5 days (184%, P < 0.001) but not 10 days of exercise. NT-4/5 and trkB were not affected at either time-point. BDNF mRNA was significantly higher in soleus at rest when compared with medial gastrocnemius (193%, P < 0.05). No significant effects of muscle type were detected for NT-4/5 and trkB. Our results indicate differential control of BDNF expression between soleus and medial gastrocnemius following 5 days of exercise. BDNF may be a protein with an uncharacterized contribution to the acute adaptation of skeletal muscle to exercise, whereas NT-4/5 shows no response. Muscle Nerve, 2009

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