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Sequencing protocols to genotype mdx, mdx4cv, and mdx5cv mice

Authors

  • Glen B. Banks PhD,

    1. Department of Neurology, University of Washington, Mail Stop 357720, 1959 NE Pacific St., Seattle, Washington 98195, USA
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  • Ariana C. Combs BS,

    1. Department of Neurology, University of Washington, Mail Stop 357720, 1959 NE Pacific St., Seattle, Washington 98195, USA
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  • Jeffrey S. Chamberlain PhD

    Corresponding author
    1. Department of Neurology, University of Washington, Mail Stop 357720, 1959 NE Pacific St., Seattle, Washington 98195, USA
    2. Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    3. Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    • Department of Neurology, University of Washington, Mail Stop 357720, 1959 NE Pacific St., Seattle, Washington 98195, USA
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Abstract

Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx, mdx4cv, and mdx5cv mice. This method clearly distinguishes between wildtype, heterozygous, and mutant transcripts, and thereby time and money can be saved by avoiding false positives. Muscle Nerve, 2010

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