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Short Report
Genotyping mdx, mdx3cv, and mdx4cv mice by primer competition polymerase chain reaction
Article first published online: 9 DEC 2010
DOI: 10.1002/mus.21873
Copyright © 2010 Wiley Periodicals, Inc.
Additional Information
How to Cite
Shin, J.-H., Hakim, C. H., Zhang, K. and Duan, D. (2011), Genotyping mdx, mdx3cv, and mdx4cv mice by primer competition polymerase chain reaction. Muscle & Nerve, 43: 283–286. doi: 10.1002/mus.21873
Publication History
- Issue published online: 19 JAN 2011
- Article first published online: 9 DEC 2010
- Manuscript Accepted: 30 JUL 2010
- Abstract
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Keywords:
- Duchenne muscular dystrophy;
- Genotyping;
- mdx;
- mdx3cv;
- mdx4cv;
- PCR
Abstract
mdx, mdx3cv, and mdx4cv mice are among the most commonly used models for the study of Duchenne muscular dystrophy. Their disease is caused by point mutations in the dystrophin gene. Despite widespread use of these models, genotyping has not always been straightforward. Current methods require multiple polymerase chain reactions (PCRs), post-PCR manipulations, and/or special equipment/reagents. Herein we report a simple, robust PCR genotyping method based on primer competition. This approach could also be applied in genotyping other point-mutation models. Muscle Nerve , 2010