PAX7+ satellite cells in young and older adults following resistance exercise

Authors

  • Dillon K. Walker PhD,

    1. Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas, USA
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  • Christopher S. Fry PhD,

    1. Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas, USA
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  • MICAH J. Drummond PhD,

    1. Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas, USA
    3. Sealy Center on Aging, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
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  • Jared M. Dickinson PhD,

    1. Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas, USA
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  • Kyle L. Timmerman PhD,

    1. Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas, USA
    3. Sealy Center on Aging, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
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  • David M. Gundermann MSc,

    1. Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas, USA
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  • Kristofer Jennings PhD,

    1. Department of Preventative Medicine and Community Health, Division of Epidemiology and Biostatistics, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
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  • Elena Volpi MD, PhD,

    1. Sealy Center on Aging, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Department of Internal Medicine, Division of Geriatrics, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
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  • Blake B. Rasmussen PhD

    Corresponding author
    1. Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    2. Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas, USA
    3. Sealy Center on Aging, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
    • Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA
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Abstract

Introduction:

Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known.

Methods:

Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27Kip1 and cyclin D1.

Results:

Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27Kip1.

Conclusions:

An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h. Muscle Nerve 46: 51–59, 2012

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