Novel role for prepatterned nicotinic acetylcholine receptors during myogenesis
Article first published online: 29 MAY 2012
Copyright © 2012 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 46, Issue 1, pages 112–121, July 2012
How to Cite
Bernareggi, A., Luin, E., Formaggio, E., Fumagalli, G. and Lorenzon, P. (2012), Novel role for prepatterned nicotinic acetylcholine receptors during myogenesis. Muscle Nerve, 46: 112–121. doi: 10.1002/mus.23284
- Issue published online: 12 JUN 2012
- Article first published online: 29 MAY 2012
- Accepted manuscript online: 6 JAN 2012 08:40AM EST
- Manuscript Accepted: 18 DEC 2011
- nicotinic acetylcholine receptors;
- prepatterned clusters;
- skeletal muscle
Before the nerve contacts the skeletal muscle, the nicotinic acetylcholine receptors (nAChRs) form aggregates known as prepatterned clusters. We investigated their role in the occurrence of Ca2+ spikes and twitching during myogenesis.
Cultured mouse myotubes were used as cell models. Cells were subjected to a combination of immunostaining, Ca2+ imaging and electrophysiological analysis.
A single prepatterned nAChR cluster per myotube was generally detected. A correlation between formation of the prepatterned clusters and occurrence of Ca2+ spikes and twitching was observed. Increase in size of the prepatterned clusters raised the frequency of Ca2+ spikes and twitching. Blockade of the electrical activity triggered by the autocrine activation of prepatterned nAChR induced over-numbered nAChR clusters.
Prepatterned nAChR aggregation is required for Ca2+ spikes and twitching of developing myotubes. Moreover, prepatterned nAChR-driven electrical activity preserves the distribution of nAChRs, mimicking the effect of synaptic activity before innervation. Muscle Nerve 46: 112–121, 2012