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GDNF released from microspheres enhances nerve regeneration after delayed repair

Authors

  • Matthew D. Wood PhD,

    Corresponding author
    1. Division of Plastic and Reconstructive Surgery, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada, M5G 1X8
    2. Department of Physiology and Experimental Medicine, The Hospital for Sick Children Research Institute, Elizabeth McMaster Building, Toronto, ON, Canada
    • Division of Plastic and Reconstructive Surgery, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada, M5G 1X8
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  • Howard Kim PhD,

    1. Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, Canada
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  • Alex Bilbily BS,

    1. Department of Physiology and Experimental Medicine, The Hospital for Sick Children Research Institute, Elizabeth McMaster Building, Toronto, ON, Canada
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  • Stephen W.P. Kemp PhD,

    1. Division of Plastic and Reconstructive Surgery, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada, M5G 1X8
    2. Department of Physiology and Experimental Medicine, The Hospital for Sick Children Research Institute, Elizabeth McMaster Building, Toronto, ON, Canada
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  • Christine Lafontaine MS,

    1. Division of Plastic and Reconstructive Surgery, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada, M5G 1X8
    2. Department of Physiology and Experimental Medicine, The Hospital for Sick Children Research Institute, Elizabeth McMaster Building, Toronto, ON, Canada
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  • Tessa Gordon PhD,

    1. Division of Plastic and Reconstructive Surgery, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada, M5G 1X8
    2. Department of Physiology and Experimental Medicine, The Hospital for Sick Children Research Institute, Elizabeth McMaster Building, Toronto, ON, Canada
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  • Molly S. Shoichet PhD,

    1. Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, Canada
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  • Gregory H. Borschel MD

    1. Division of Plastic and Reconstructive Surgery, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada, M5G 1X8
    2. Department of Physiology and Experimental Medicine, The Hospital for Sick Children Research Institute, Elizabeth McMaster Building, Toronto, ON, Canada
    3. Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, Canada
    4. Department of Surgery, University of Toronto, 100 College Street, Toronto, Ontario, Canada
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Abstract

Introduction: Delays in surgical repair following nerve transection produce progressively inferior motor nerve regeneration. Regeneration can be improved with delivery of exogenous growth factor. We developed a delivery system that could be applied at the nerve repair site to deliver growth factors locally to regenerating nerve. Methods: Poly(lactic-co-glycolic acid) microspheres containing glial-derived neurotrophic factor (GDNF) suspended within fibrin were developed into a delivery system for local application surrounding nerve at a repair site in an experimental rat model. Results: The system containing GDNF remained at the injury site for up to 2 weeks and improved motor nerve regeneration following chronic axotomy and denervation. Conclusions: Based on the positive outcome of the delivery system, we plan to study the delivery system over longer time courses of release and nerve regeneration. Muscle Nerve 46: 122–124, 2012

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