Research Article

IgG anti-Galnac-GD1a antibodies bind to neuromuscular junctions of rat hemidiaphragm

  1. Takumi Nagaoka MS1,2,
  2. Sayako Hotta MS1,
  3. Terumasa Chiba BSc1,
  4. Iku Utsunomiya PhD3,
  5. Kenji Abe PhD4,
  6. Hiide Yoshino MD PhD5,
  7. Chiaki Koshikawa MS2,
  8. Kyoji Taguchi PhD1,*

Article first published online: 10 OCT 2012

DOI: 10.1002/mus.23385

Issue

Muscle & Nerve

Muscle & Nerve

Volume 46, Issue 5, pages 705–710, November 2012

Additional Information

How to Cite

Nagaoka, T., Hotta, S., Chiba, T., Utsunomiya, I., Abe, K., Yoshino, H., Koshikawa, C. and Taguchi, K. (2012), IgG anti-Galnac-GD1a antibodies bind to neuromuscular junctions of rat hemidiaphragm. Muscle Nerve, 46: 705–710. doi: 10.1002/mus.23385

Author Information

  1. 1

    Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan

  2. 2

    Department of Pharmaceuticals, Dokkyo University School of Medicine, Mibu, Tochigi, Japan

  3. 3

    Department of Pharmacotherapics, Showa Pharmaceutical University, Machida, Tokyo, Japan

  4. 4

    Department of Pharmacology, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima, Japan

  5. 5

    Department of Neurology, Kohnodai Hospital, National Center for Global Health and Medicine, Ichikawa, Chiba, Japan

*Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan

Publication History

  1. Issue published online: 10 OCT 2012
  2. Article first published online: 10 OCT 2012
  3. Accepted manuscript online: 16 MAR 2012 01:49AM EST
  4. Manuscript Accepted: 12 MAR 2012

Funded by

  • Ministry of Education, Science and Culture of Japan

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Keywords:

  • hemidiaphragm;
  • IgG anti–GalNAc-GD1a antibodies;
  • neuromuscular junctions;
  • nerve terminal;
  • ventral root

Abstract

Introduction: We investigated the localization of a ganglioside, N-acetylgalactosaminyl GD1a (GalNAc-GD1a), in peripheral nerves with an IgG anti–GalNAc-GD1a antibody, which was produced in rabbits immunized with GalNAc-GD1a. Methods: Teased fibers from ventral and dorsal roots and hemidiaphragm sections of rats were assessed using fluorescent double- and triple-labeling methods. Results: The nodal and paranodal regions of teased fibers from ventral roots were immunostained with IgG anti–GalNAc-GD1a antibodies. After collagenase treatment, no staining was seen with IgG anti–GalNAc-GD1a or anti-NF200 antibodies, whereas α-bungarotoxin selectively stained nerve terminals. In cross-sectional and longitudinal sections of rat hemidiaphragm, IgG anti–GalNAc-GD1a antibodies overlapped with α-BuTx and anti-NF200 antibodies, indicating that GalNAc-GD1a is localized to the nerve terminal. IgG anti–GalNAc-GD1a antibody staining also overlapped with that of AChR clusters and syntaxin-positive presynaptic nerve terminals. Conclusion: GalNAc-GD1 is localized in both pre- and postsynaptic nerve terminals of neuromuscular junctions. Muscle Nerve 46: 705–710, 2012

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