IgG anti-Galnac-GD1a antibodies bind to neuromuscular junctions of rat hemidiaphragm

Authors

  • Takumi Nagaoka MS,

    1. Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan
    2. Department of Pharmaceuticals, Dokkyo University School of Medicine, Mibu, Tochigi, Japan
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  • Sayako Hotta MS,

    1. Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan
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  • Terumasa Chiba BSc,

    1. Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan
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  • Iku Utsunomiya PhD,

    1. Department of Pharmacotherapics, Showa Pharmaceutical University, Machida, Tokyo, Japan
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  • Kenji Abe PhD,

    1. Department of Pharmacology, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima, Japan
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  • Hiide Yoshino MD PhD,

    1. Department of Neurology, Kohnodai Hospital, National Center for Global Health and Medicine, Ichikawa, Chiba, Japan
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  • Chiaki Koshikawa MS,

    1. Department of Pharmaceuticals, Dokkyo University School of Medicine, Mibu, Tochigi, Japan
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  • Kyoji Taguchi PhD

    Corresponding author
    1. Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan
    • Department of Medicinal Pharmacology, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan
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Abstract

Introduction: We investigated the localization of a ganglioside, N-acetylgalactosaminyl GD1a (GalNAc-GD1a), in peripheral nerves with an IgG anti–GalNAc-GD1a antibody, which was produced in rabbits immunized with GalNAc-GD1a. Methods: Teased fibers from ventral and dorsal roots and hemidiaphragm sections of rats were assessed using fluorescent double- and triple-labeling methods. Results: The nodal and paranodal regions of teased fibers from ventral roots were immunostained with IgG anti–GalNAc-GD1a antibodies. After collagenase treatment, no staining was seen with IgG anti–GalNAc-GD1a or anti-NF200 antibodies, whereas α-bungarotoxin selectively stained nerve terminals. In cross-sectional and longitudinal sections of rat hemidiaphragm, IgG anti–GalNAc-GD1a antibodies overlapped with α-BuTx and anti-NF200 antibodies, indicating that GalNAc-GD1a is localized to the nerve terminal. IgG anti–GalNAc-GD1a antibody staining also overlapped with that of AChR clusters and syntaxin-positive presynaptic nerve terminals. Conclusion: GalNAc-GD1 is localized in both pre- and postsynaptic nerve terminals of neuromuscular junctions. Muscle Nerve 46: 705–710, 2012

Ancillary