Altered autophagy gene expression and persistent atrophy suggest impaired remodeling in chronic hemiplegic human skeletal muscle
Article first published online: 19 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 46, Issue 5, pages 785–792, November 2012
How to Cite
von Walden, F., Jakobsson, F., EdstrÖm, L. and Nader, G. A. (2012), Altered autophagy gene expression and persistent atrophy suggest impaired remodeling in chronic hemiplegic human skeletal muscle. Muscle Nerve, 46: 785–792. doi: 10.1002/mus.23387
- Issue published online: 10 OCT 2012
- Article first published online: 19 SEP 2012
- Accepted manuscript online: 20 MAR 2012 09:39AM EST
- Manuscript Accepted: 13 MAR 2012
- skeletal muscle;
Upper motor neuron lesions after stroke are a major cause of disability. We aimed to determine whether skeletal muscles from these patients display typical molecular signatures of inflammation, growth arrest, and atrophy.
Muscle biopsies were analyzed for morphological, histochemical, ultrastructural, and molecular features indicative of changes in gene expression involved in muscle atrophy.
Chronic hemiplegia resulted in ∽9.5% atrophy, fiber type shifts, and histochemical and ultrastructural signs of impaired remodeling. TNF and TWEAK expressions were unaltered, but MSTN mRNA was lower (−73%, P < 0.05) in paretic tibialis anterior vs. age-matched controls. The expression of autophagy-related genes (BCN-1, LC3, and GABARAPL1) was lower in paretic tibialis anterior (−81%, −48%, and −60%, respectively, P < 0.01) and soleus (−85%, −54%, and −60% respectively, P < 0.01) compared with old controls.
Persistent atrophy in chronic spastic hemiplegia may be associated with impaired remodeling partly due to altered autophagy gene expression. Muscle Nerve, 2012