Nerve allografts supplemented with schwann cells overexpressing glial-cell-line–derived neurotrophic factor
Article first published online: 21 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 47, Issue 2, pages 213–223, February 2013
How to Cite
Santosa, K. B., Jesuraj, N. J., Viader, A., MacEwan, M., Newton, P., Hunter, D. A., Mackinnon, S. E. and Johnson, P. J. (2013), Nerve allografts supplemented with schwann cells overexpressing glial-cell-line–derived neurotrophic factor. Muscle Nerve, 47: 213–223. doi: 10.1002/mus.23490
- Issue published online: 24 JAN 2013
- Article first published online: 21 NOV 2012
- Accepted manuscript online: 11 JUN 2012 12:12PM EST
- Manuscript Accepted: 3 JUN 2012
- lentiviral vector;
- nerve regeneration;
- neurotrophic factor;
- peripheral nerve injury;
- Schwann cells
We sought to determine whether supplementation of acellular nerve allografts (ANAs) with Schwann cells overexpressing GDNF (G-SCs) would enhance functional recovery after peripheral nerve injury.
SCs expanded in vitro were infected with a lentiviral vector to induce GDNF overexpression. Wild-type SCs (WT-SCs) and G-SCs were seeded into ANAs used to repair a 14-mm nerve gap defect. Animals were harvested after 6 and 12 weeks for histomorphometric and muscle force analysis.
At 6 weeks, histomorphometry revealed that ANAs supplemented with G-SCs promoted similar regeneration compared with isograft at midgraft. However, G-SCs failed to promote regeneration into the distal stump. At 12 weeks, ANAs with G-SCs had lower maximum and specific force production compared with controls.
The combined results suggest that consistent overexpression of GDNF by G-SCs trapped axons in the graft and prevented functional regeneration. Muscle Nerve, 2013