Validation of the modified LDIFlare technique: A simple and quick method to assess C-fiber function
Article first published online: 21 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc., a Wiley company
Muscle & Nerve
Volume 47, Issue 3, pages 351–356, March 2013
How to Cite
Vas, P. R.J. and Rayman, G. (2013), Validation of the modified LDIFlare technique: A simple and quick method to assess C-fiber function. Muscle Nerve, 47: 351–356. doi: 10.1002/mus.23532
- Issue published online: 23 FEB 2013
- Article first published online: 21 NOV 2012
- Accepted manuscript online: 23 JUL 2012 06:16AM EST
- Manuscript Accepted: 17 JUL 2012
- C-fiber function;
- diabetic neuropathy;
- laser Doppler imager;
- modified LDIflare;
- microvascular responses
In this study we validated a modified laser Doppler imager method (mLDIf) for assessing C-fiber function and compared it to the original (oLDIf). Both measure flare size in foot skin after heating, but the mLDIf uses 47°C (vs. 44°C), making it quicker and better suited for clinical use.
To confirm that mLDIf assesses C-fiber function, 5 healthy controls (HC) were studied before and after local anesthesia (LA). Reproducibility and comparison with oLDIf was assessed in HC (n = 16). Finally, diabetes subjects with (DN+, n = 10) and without (DN−, n = 16) neuropathy were studied.
LA almost abolished the flare (9.3 ± 3.0 cm2 vs. 1.7 ± 0.3 cm2, P < 0.0001). mLDIf produced larger flares (9.9 ± 3.4 vs. 5.7 ± 2.3 cm2, P < 0.0001), but correlated with oLDIf (r = 0.81, P < 0.001). mLDIf was reduced in DN− (6.8 ± 2.8 vs. HC, P = 0.003), markedly so in DN+ (2.0 ± 1.1 vs. HC and DN−, P < 0.0001).
The mLDIf is a quick, practical method for assessing C-fiber function in the clinical setting. Muscle Nerve 47:351-356, 2013