Immediate force loss after eccentric contractions is increased with l-name administration, a nitric oxide synthase inhibitor
Article first published online: 24 JAN 2013
Copyright © 2012 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 47, Issue 2, pages 271–273, February 2013
How to Cite
Corona, B. T. and Ingalls, C. P. (2013), Immediate force loss after eccentric contractions is increased with l-name administration, a nitric oxide synthase inhibitor. Muscle Nerve, 47: 271–273. doi: 10.1002/mus.23655
- Issue published online: 24 JAN 2013
- Article first published online: 24 JAN 2013
- Accepted manuscript online: 28 AUG 2012 03:27AM EST
- Manuscript Accepted: 24 AUG 2012
- National Institute of Arthritis and Musculoskeletal and Skin Diseases. Grant Number: AR-41802
- eccentric contractions;
- muscle injury;
- nitric oxide synthase
Nitric oxide (NO) signaling regulates many biological processes in skeletal muscle, wherein aberrant signaling contributes to myopathic conditions (e.g., Duchenne muscular dystrophy). NO has been shown to play a role in muscle regeneration after injury. However, less is known about its role during injury. In this study we aimed to determine whether NO synthase (NOS) inhibition exacerbates functional deficits immediately after the performance of eccentric contractions.
Wild-type mouse extensor digitorum longus (EDL) muscles underwent in vitro functional testing in the presence or absence of a non-specific NOS inhibitor (L-NAME, 10 mM) before and after performance of 10 eccentric contractions.
After eccentric contractions, Po was reduced by ∽25% for muscle in regular physiological solution but by ∽50% with the addition of L-NAME (P = 0.009).
Non-specific blockade of NOS exacerbates functional deficits immediately after eccentric contractions, suggesting that NO signaling protects skeletal muscle from excessive injury in healthy muscle. Muscle Nerve, 2013