This article includes Supplementary Material available via the internet at http://www.mrw.interscience.wiley.com/suppmat/0148-639X/suppmat/
Optical inhibition of motor nerve and muscle activity in vivo
Article first published online: 30 APR 2013
Copyright © 2012 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 47, Issue 6, pages 916–921, June 2013
How to Cite
Liske, H., Towne, C., Anikeeva, P., Zhao, S., Feng, G., Deisseroth, K. and Delp, S. (2013), Optical inhibition of motor nerve and muscle activity in vivo. Muscle Nerve, 47: 916–921. doi: 10.1002/mus.23696
Supported by a Stanford Bio-X Interdisciplinary Initiatives Award, a Stanford National Institutes of Health Graduate Training Program in Biotechnology grant, a grant from the W.M. Keck Foundation, and NIH grant R01NS080954.
- Issue published online: 23 MAY 2013
- Article first published online: 30 APR 2013
- Accepted manuscript online: 14 NOV 2012 06:10AM EST
- Manuscript Accepted: 7 OCT 2012
- sciatic nerve;
- spastic hypertonia
Introduction: There is no therapeutic approach that provides precise and rapidly reversible inhibition of motor nerve and muscle activity for treatment of spastic hypertonia. Methods: We used optogenetics to demonstrate precise and rapidly reversible light-mediated inhibition of motor nerve and muscle activity in vivo in transgenic Thy1::eNpHR2.0 mice. Results: We found optical inhibition of motor nerve and muscle activity to be effective at all muscle force amplitudes and determined that muscle activity can be modulated by changing light pulse duration and light power density. Conclusions: This demonstration of optical inhibition of motor nerves is an important advancement toward novel optogenetics-based therapies for spastic hypertonia. Muscle Nerve 47: 916–921, 2013