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mus23768-sup-0001-suppfig1.doc247KFIGURE S1. BMSC (A) or uBMSClacZ+(B, C) were fixed. Cells were treated under oxidative conditions, in the presence of the substrate X-Gal, an assay for the β-galactosidase enzyme, expressed by the reporter gene lacZ(A, B) or submitted to indirect immunofluorescence with anti–β-galactosidase detected by Alexa 488 (C). The expression of the reporter gene and the enzymatic activity of the protein β-galactosidase are detected by the blue color (B), as opposed to the negative control (A). Scale bars: 50 μm.
mus23768-sup-0002-suppfig2.doc211KFIGURE S2. dBMSClacZ+ expresses the reporter gene lacZ and Schwann cell markers. uBMSClacZ+ were submitted to the full protocol for differentiation into Schwann-like cells followed by immunofluorescence analyses. Double labeling was performed with anti–β-galactosidase antibody detected by Alexa 488 (green) (A, D, G) and antibodies directed to Schwann cell progenitors (Oct-6) (B), mature Schwann cells and progenitors (p75NTR) (E), and mature Schwann cells (S100) (H), detected by Alexa 568, in red. Panels (C), (F), and (I) are due to the overlapping of images, respectively, between panels (A) and (B), (D) and (E), and (G) and (H). Overlapping images show that cells expressing the reporter protein β-galactosidase are the same that express the markers for Schwann cell lineage. Images were obtained after z sectioning in a confocal microscope (LSM410; Zeiss, Germany). Scale bars: 25 μm (A–C, G–I); 50 μm (D–F).
mus23768-sup-0003-suppfig3.doc737KFIGURE S3. Examples of CMAP profiles obtained from the mandibular branch of the facial nerve from groups A (A, E, I), B (B, F, J), C (C, G, K), and D (D, H, L) in the following 3 time periods: before neurotmesis (column 1), and 3 and 6 weeks after surgery (columns 1 and 2), respectively.
mus23768-sup-0004-suppinfo.doc33KSupplementary Material Information.

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