This study was supported by grants from the INCT Program Project of the National Council for Scientific and Technological Development, CNPq, Brasília, Brazil (573633/2008-8 to L.A.H. and R.F.B.) and the São Paulo Research Foundation, FAPESP (CEPID 1998/14254-2), through the facilities of the Human Genome Research Center (Instituto de Biociências, USP, São Paulo, Brazil). Research was funded by FAPESP (2008/53857-8 to L.A.H. and 2008/00458-9 to R.F.B.).
Bone marrow stem cells in facial nerve regeneration from isolated stumps
Version of Record online: 3 JUL 2013
Copyright © 2013 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 48, Issue 3, pages 423–429, September 2013
How to Cite
Salomone, R., Bento, R. F., Costa, H. J.Z.R., Azzi-Nogueira, D., Ovando, P. C., Da-Silva, C. F., Zanatta, D. B., Strauss, B. E. and Haddad, L. A. (2013), Bone marrow stem cells in facial nerve regeneration from isolated stumps. Muscle Nerve, 48: 423–429. doi: 10.1002/mus.23768
- Issue online: 27 AUG 2013
- Version of Record online: 3 JUL 2013
- Accepted manuscript online: 30 JAN 2013 10:55PM EST
- Manuscript Accepted: 24 DEC 2012
Additional Supporting Information may be found in the online version of this article.
|mus23768-sup-0001-suppfig1.doc||247K||FIGURE S1. BMSC (A) or uBMSClacZ+(B, C) were fixed. Cells were treated under oxidative conditions, in the presence of the substrate X-Gal, an assay for the β-galactosidase enzyme, expressed by the reporter gene lacZ(A, B) or submitted to indirect immunofluorescence with anti–β-galactosidase detected by Alexa 488 (C). The expression of the reporter gene and the enzymatic activity of the protein β-galactosidase are detected by the blue color (B), as opposed to the negative control (A). Scale bars: 50 μm.|
|mus23768-sup-0002-suppfig2.doc||211K||FIGURE S2. dBMSClacZ+ expresses the reporter gene lacZ and Schwann cell markers. uBMSClacZ+ were submitted to the full protocol for differentiation into Schwann-like cells followed by immunofluorescence analyses. Double labeling was performed with anti–β-galactosidase antibody detected by Alexa 488 (green) (A, D, G) and antibodies directed to Schwann cell progenitors (Oct-6) (B), mature Schwann cells and progenitors (p75NTR) (E), and mature Schwann cells (S100) (H), detected by Alexa 568, in red. Panels (C), (F), and (I) are due to the overlapping of images, respectively, between panels (A) and (B), (D) and (E), and (G) and (H). Overlapping images show that cells expressing the reporter protein β-galactosidase are the same that express the markers for Schwann cell lineage. Images were obtained after z sectioning in a confocal microscope (LSM410; Zeiss, Germany). Scale bars: 25 μm (A–C, G–I); 50 μm (D–F).|
|mus23768-sup-0003-suppfig3.doc||737K||FIGURE S3. Examples of CMAP profiles obtained from the mandibular branch of the facial nerve from groups A (A, E, I), B (B, F, J), C (C, G, K), and D (D, H, L) in the following 3 time periods: before neurotmesis (column 1), and 3 and 6 weeks after surgery (columns 1 and 2), respectively.|
|mus23768-sup-0004-suppinfo.doc||33K||Supplementary Material Information.|
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